1887

Abstract

Acid phosphatase, esterases, and glutamate and malate dehydrogenases of 192 strains of were analysed by horizontal polyacrylamide agarose gel electrophoresis and by isoelectrofocusing in thin-layer polyacrylamide gels. The six species were clearly separated from each other by their distinct enzyme electrophoretic polymorphism. For , the strains of biotype 5 were differentiated from the other biotypes by the mobility of glutamate dehydrogenase. For , six zymotypes were delineated by pI and by the mobility of the enzymes. Variation in number or mobility of esterases within each species could represent a marker for epidemiological and ecological analyses. A linear relationship was obtained between the mean genetic diversity coefficient of enzymes and the mean percentage DNA-DNA relatedness of and

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1988-02-01
2021-08-01
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References

  1. Baker P.M., Farmer J.J.III 1982; New bacteriophage typing system for Yersinia enterocolitica, Yersinia kristensenii, Yersinia frederiksenii and Yersinia intermedia. Correlation with serotyping, biotyping and antibiotic susceptibility 15:491–502
    [Google Scholar]
  2. Baptist J.N., Shaw C.R., Mandel M. 1969; Zone electrophoresis of enzymes in bacterial taxonomy. Journal of Bacteriology 99:180–188
    [Google Scholar]
  3. Bercovier H., Mollaret H.H. 1984; Yersinia. In Bergey’s Manual of Systematic Bacteriology pp. 498–506 Edited by Krieg N. R. Baltimore: Williams & Wilkins;
    [Google Scholar]
  4. Bercovier H., Brault J., Barre N., Treignier M., Alonso J.M., Mollaret H.H. 1978; Biochemical, serological and phage typing characteristics of 459 Yersinia strains isolated from a terrestrial ecosystem. Current Microbiology 1:353–357
    [Google Scholar]
  5. Bercovier H., Ursing J., Brenner D.J., Steigerwalt A.G., Fanning G.R., Carter G.P., Mollaret H.H. 1980; Yersinia kristensenii: a new species of Enterobacteriaceae composed of sucrosenegative strains (formerly called atypical Yersinia enterocolitica or Yersinia enterocolitica-like). Current Microbiology 4:219–224
    [Google Scholar]
  6. Bercovier H., Steigerwalt A.G., Guiyoule A., Huntley-Carter G., Brenner D.J. 1984; Yersinia aldovae (formerly Yersinia enterocolitica-like group X2): a new species of Enterobacteriaceae isolated from aquatic ecosystems. International Journal of Systematic Bacteriology 34:166–172
    [Google Scholar]
  7. Brenner D.J., Ursing J., Bercovier H., Steiger-Walt A.G., Fanning G.R., Alonso J.M., Mollaret H.H. 1980a; Deoxyribonucleic acid relatedness in Yersinia enterocolitica and Yersinia enterocolitica-like organisms. Current Microbiology 4:195–200
    [Google Scholar]
  8. Brenner D.J., Bercovier H., Ursing J., Alonso J.M., Steigerwalt A.G., Fanning G.R., Carter G.P., Mollaret H.H. 1980b; Yersinia intermedia: a new species of Enterobacteriaceae composed of rhamnose-positive, melibiose positive, raffinose-positive strains (formerly called Yersinia enterocolitica or Yersinia enterocolitica-hke). Current Microbiology 4:207–212
    [Google Scholar]
  9. Goullet PH. 1980; Distinctive electrophoretic patterns of esterases from Klebsiella pneumoniae, K oxytoca, Enterobacter aerogenes and E. gergoviae. Journal of General Microbiology 117:483–491
    [Google Scholar]
  10. Goullet PH., Picard B. 1984a; Distinctive electrophoretic and isoelectric focusing patterns of esterases from Yersinia enterocolitica and Yersinia pseudotuberculosis. Journal of General Microbiology 130:1471–1480
    [Google Scholar]
  11. Goullet PH., Picard B. 1984a; Typage électro phorétique des esterases d’Escherichia coli au cours de septicémies. Presse médicale 13:1079–1081
    [Google Scholar]
  12. Goullet PH., Picard B. 1985a; A two-dimensional electrophoretic profile for bacterial esterases. Electrophoresis 6:132–135
    [Google Scholar]
  13. Goullet PH., Picard B. 1985b; Etude du polymorphisme électrophorétique des lactate-, malate- et glutamate-deshydrogenases, de la phosphatase acide et des esterases de Providenciaalc alifaciens, P.stuartii et P. rustigianii. Annales de l’Institut Pasteur/Microbiologie 136A:347–358
    [Google Scholar]
  14. Goullet PH., Picard B. 1986; Characterization of Enterobacter cloacae and E.sakazakii by electrophoretic polymorphism of acid phosphatase, esterases, and glutamate, lactate and malate dehydrogenases. Journal of General Microbiology 132:3105–3112
    [Google Scholar]
  15. Lennox E.S. 1955; Transduction of linked characters of the host by bacteriophage PI. Virology 1:190–206
    [Google Scholar]
  16. Nei M. 1978; Estimation of average heterozygosity and genetic distance from a small sample of individuals. Genetics 89:583–590
    [Google Scholar]
  17. Picard B., Goullet PH. 1985; Comparative electrophoretic profiles of esterases, and of glutamate, lactate and malate dehydrogenases, from Aeromonashydrophila, A, caviae and A. sobria. Journal of General Microbiology 131:3385–3391
    [Google Scholar]
  18. Picard B., Krishnamoorthy R., Goullet PH. 1987; Enzyme polymorphism in bacteria: study of molecular relatedness by combined isoelectric focus- ing-electrophoresis. Electrophoresis 8:149–152
    [Google Scholar]
  19. Selander R.K., Caugant D.A., Ochman H., Musser J.M., Gilmour M.N., Whittam T.S. 1986; Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Applied and Environmental Microbiology 51:873–884
    [Google Scholar]
  20. Siciliano M., Shaw C.R. 1976; Separation and visualization of enzymes on gels. In Chromatographic and Electrophoretic Techniques, 4th edn. pp. 185–209 Edited by Smith I. Chicago: Yearbook Medical Publishers;
    [Google Scholar]
  21. Uriel J. 1963; Caracterisation d’enzymes par électrophorèse en gélose. In Technique de Laboratoire, pp. 930–935 Edited by Loiseleur J. Paris: Masson;
    [Google Scholar]
  22. Ursing J., Brenner D.J., Bercovier H., Fanning R., Steigerwalt A.G., Brault J., Mollaret H.H. 1980; Yersinia frederiksenii: a new species of Enterobacteriaceae composed of rhamnose-positive strains (formerly called atypical Yersinia enterocolitica or Yersinia enterocolitica-like). Current Microbiology 4:213–217
    [Google Scholar]
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