1887

Abstract

The utilization of exogenous nicotinamide adenine dinucleotide (NAD) by was studied in suspensions of whole cells using radiolabelled NAD, nicotinamide mononucleotide (NMN), and nicotinamide ribonucleoside (NR). The utilization of these compounds by has the following characteristics. (1) NAD is not taken up intact, but rather is degraded to NMN or NR prior to internalization. (2) Uptake is carrier-mediated and energy-dependent with saturation kinetics. (3) There is specificity for the -configuration of the glycopyridine linkage. (4) An intact carboxamide group is required on the pyridine ring. The intracellular metabolism of NAD was studied in crude cell extracts and in whole cells using carbonyl-C-labelled NR, NMN, NAD, nicotinamide, and nicotinic acid as substrates in separate experiments. A synthetic pathway from NR through NMN to NAD that requires Mg and ATP was demonstrated. Nicotinamide was found as an end-product of NAD degradation. Nicotinic acid mononucleotide and nicotinic acid adenine dinucleotide were not found as intermediates. The NAD synthetic pathway in differs from the Preiss-Handler pathway and the pyridine nucleotide cycles described in other bacteria.

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1988-10-01
2024-05-04
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References

  1. Bachur N.R., Kaplan N.O. 1955; Metabolism of diphosphopyridine nucleotide and related compounds in Hemophilus parainfluenzae (abstract).. Bacteriological Proceedings 55:116
    [Google Scholar]
  2. Chandler J.R., Gholson R.K. 1972; De novo biosynthesis of nicotinamide adenine dinucleotide in Escherichia coli\ excretion of quinolinic acid by mutants lacking quinolinate phosphoribosyl transferase.. Journal of Bacteriology 111:98–102
    [Google Scholar]
  3. Cruz L.J., Salabao J.L., Flores E., Olivera B.M. 1974; Pyridine nucleotide metabolism: purification of a nicotinamide mononucleotide- specific deaminase.. Technical Report no.18 UP Natural Science Research Center, Quezon City, Philippines.
    [Google Scholar]
  4. Cynamon M.H., Sorg T.B., Patapow A. 1985; Utilization of exogenous nicotinamide adenine dinucleotide by Hemophilus parainfluenza.. Clinical Research 33:398A
    [Google Scholar]
  5. Foster J.W. 1981; Pyridine nucleotide cycle of Salmonella typhimurium: in vitro demonstration of nicotinamide adenine dinucleotide glycohydrolase, nicotinamide mononucleotide glycohydrolase, and nicotinamide adenine dinucleotide pyrophosphatase activities.. Journal of Bacteriology 145:1002–1009
    [Google Scholar]
  6. Foster J.W., Moat A.G. 1980; Nicotinamide adenine dinucleotide biosynthesis and pyridine nucleotide cycle metabolism in microbial systems.. Microbiological Reviews 44:83–105
    [Google Scholar]
  7. Herriot R.M., Meyer E.Y., Vogt M., Modan M. 1970; Defined medium for the growth of Haemophilus influenzae.. Journal of Bacteriology 101:513–516
    [Google Scholar]
  8. Hillyard D., Rechsteiner M., Manlapaz-Ramos P., Imperial J.S., Cruz L.J., Olivera B.M. 1981; The pyridine nucleotide cycle.. Journal of Biological Chemistry 256:8491–8497
    [Google Scholar]
  9. Hughes K.T., Cookson B.T., Ladika D., Olivera B.M., Roth J.R. 1983; 6-Aminonicotinamide- resistant mutants of Salmonella typhimurium.. Journal of Bacteriology 154:1126–1136
    [Google Scholar]
  10. Kahn D.W., Anderson B.M. 1986; Characterization of Haemophilus influenzae nucleotide pyrophosphatase.. Journal of Biological Chemistry 261:6016–6025
    [Google Scholar]
  11. Kasarov L.B., Moat A.G. 1972; Convenient method for enzymatic synthesis of 14C-nicotinamide riboside.. Analytical Biochemistry 46:181–186
    [Google Scholar]
  12. Kay W.W. 1971; Two aspartate transport systems in Escherichia coli.. Journal of Biological Chemistry 245:7373–7382
    [Google Scholar]
  13. Killian M. 1980; Haemophilus.. In Manual of Clinical Microbiology, 3rd edn. pp. 330–336 Lennette E.H., Balows A., Hausler W.J. JR Truant J.P. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  14. Lineweaver H., Burk D. 1934; The determination of enzyme dissociation constants.. Journal of the American Chemistry Society 56:658–666
    [Google Scholar]
  15. Liu G., Foster J., Manlapaz-Ramos P., Olivera B.M. 1982; Nucleoside salvage pathway for NAD biosynthesis in Salmonella typhimurium.. Journal of Bacteriology 152:1111–1116
    [Google Scholar]
  16. Michal G. 1978; Determination of Michaelis constants and inhibitor constants.. In Principles of Enzymatic Analysis pp. 29–40 Bergmeyer H.U., Gawehn K. Edited by Weinheim, New York: Verlag Chemie;
    [Google Scholar]
  17. Miksic J.R., Brown P.R. 1977; Complementary use of the reversed-phase and anion exchange modes of high-pressure liquid chromatography for studies of reduced nicotinamide adenine dinucleotide.. Journal of Chromatography 142:641–649
    [Google Scholar]
  18. O’reilly T., Niven D.F. 1986a; Defining the metabolic and growth responses of porcine haemo- phili to exogenous pyridine nucleotides and precursors.. Journal of General Microbiology 132:807–818
    [Google Scholar]
  19. O’reilly T., Niven D.F. 1986b; Pyridine nucleotide metabolism by extracts derived from Haemophilus parasuis and H. pleuropneumoniae.. Canadian Journal of Microbiology 32:733–737
    [Google Scholar]
  20. Preiss J., Handler P. 1958a; Biosynthesis of diphosphopyridine nucleotide. I. Identification of intermediates.. Journal of Biological Chemistry 233:488–492
    [Google Scholar]
  21. Preiss J., Handler P. 1958b; Biosynthesis of diphosphopyridine nucleotide. II. Enzymatic aspects.. Journal of Biological Chemistry 233:493–500
    [Google Scholar]
  22. Sorg T., Cynamon M., Tyson P. 1986; NAD metabolism in Hemophilus influenza and parainfluenza. American Society for Microbiology Annual Meeting (86), March 1986.. Abstract D19
    [Google Scholar]
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