SUMMARY: Mutants of resistant to novobiocin (Nov-mutants) were isolated after mutagenesis of strain Challis with ethyl methanesulphonate. The resistance phenotype was transferred by DNA-mediated transformation back into the parent strain at high frequency suggesting resistance was due to mutation(s) in a single gene or in closely-linked genes. Cells of Nov-mutants had normal morphology and secreted similar proteins to the wild-type strain. However, mutant cultures had slower growth rates, the mutant cells had reduced hydrophobicity, and they showed a reduced degree of coaggregation with and . Cell envelopes prepared from Nov-mutants differed from wild-type cell envelopes in that they () were impaired in ability to coaggregate with cells, and () had altered protein composition as detected by SDS-PAGE. The results suggest that hydrophobic proteins in the cell envelope of may be necessary for coaggregation of this bacterium with actinomycetes.


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