SUMMARY: Cells of a strain of a sp., pre-grown in cadmium-free continuous culture, accumulated cadmium extensively when resuspended in a buffer that contained Cd and glycerol 2-phosphate. The accumulated compound was identified by X-ray microanalysis and magic angle spinning NMR analysis as cell-bound cadmium phosphate, probably CdHPO. Its accumulation is consistent with the activity of a phosphatase, induced during pre-growth, that continues to function in the resuspended cells to liberate HPO from glycerol 2-phosphate. This anion then combines with Cd to form insoluble cell-bound CdHPO.


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