@article{mbs:/content/journal/micro/10.1099/00221287-133-3-539, author = "Macaskie, Lynne E. and Dean, Alastair C. R. and Cheetham, Anthony K. and Jakeman, Roger J. B. and Skarnulis, A. Jerome", title = "Cadmium Accumulation by a Citrobacter sp.: the Chemical Nature of the Accumulated Metal Precipitate and its Location on the Bacterial Cells", journal= "Microbiology", year = "1987", volume = "133", number = "3", pages = "539-544", doi = "https://doi.org/10.1099/00221287-133-3-539", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-133-3-539", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Cells of a strain of a Citrobacter sp., pre-grown in cadmium-free continuous culture, accumulated cadmium extensively when resuspended in a buffer that contained Cd2+ and glycerol 2-phosphate. The accumulated compound was identified by X-ray microanalysis and magic angle spinning NMR analysis as cell-bound cadmium phosphate, probably CdHPO4. Its accumulation is consistent with the activity of a phosphatase, induced during pre-growth, that continues to function in the resuspended cells to liberate HPO2− 4 from glycerol 2-phosphate. This anion then combines with Cd2+ to form insoluble cell-bound CdHPO4.", }