1887

Abstract

To investigate the relationship between growth rate and concentration of the nutrient that limits growth, NCTC 418 () was grown in a glucose-limited chemostat. The actual time required to establish a steady-state glucose concentration exceeded that expected theoretically. Apparently, there is a long-term adaptation of the cells to nutrient limitation. As yet, it is not clear whether this has a phenotypic or genetic origin. In the final steady state, the dependence of the growth rate on glucose concentration could be mathematically described equally well by a hyperbolic and by a logarithmic function.

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/content/journal/micro/10.1099/00221287-133-2-445
1987-02-01
2022-01-17
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References

  1. Anderson FLP., Miller C. G., Roth J. R. 1976; Tandem duplications of the histidine operon observed following generalized transduction in Salmonella typhimurium . Journal of Molecular Biology 105:201–218
    [Google Scholar]
  2. Chao L., Mcbroom S. M. 1985; Evolution of transposable elements: an IS 10 insertion increases fitness in Escherichia coli . Molecular Biology and Evolution 2:359–369
    [Google Scholar]
  3. Collins S. H., Jarvis A. W., Lindsay R. J., Hamilton W. A. 1976; Proton movements coupled to lactate and alanine in Escherichia coli: isolation of mutants with altered stoichiometry in alanine transport. Journal of Bacteriology 126:1232–1244
    [Google Scholar]
  4. Cox E. C., Gibson T. C. 1974; Selection for high mutation rates in chemostats. Genetics 77:169–184
    [Google Scholar]
  5. Dam K., Van Teixeira De Mattos M. J., Westerhoff H. V. 1987; A thermodynamic view of bacterial growth. In Physiological Models (CRC Mathematical Models of Microbiology in the Press Prosser J., Bazin M. J. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  6. Dykhuizen D. E. 1978; Selection for tryptophan auxotrophs of Escherichia coli in glucose-limited chemostats as a test of the energy conservation hypothesis of evolution. Evolution 32:125–150
    [Google Scholar]
  7. Dykhuizen D. E., Hartl D. L. 1981; Evolution of competitive ability in Escherichia coli . Evolution 35:581–594
    [Google Scholar]
  8. Dykhuizen D. E., Hartl D. L. 1983; Selection in chemostats. Microbiological Reviews 47:150–168
    [Google Scholar]
  9. Evans C. G. T., Herbert D., Tempest D. W. 1970; The continuous culture of microorganisms. 2. Construction of a chemostat. Methods in Microbiology 2:211–327
    [Google Scholar]
  10. Harder W., Dijkhuizen L. 1983; Physiological responses to nutrient limitation. Annual Review of Microbiology 37:1–23
    [Google Scholar]
  11. Harder W., Kuenen J. G., Matin A. 1977; Microbial selection in continuous culture. Journal of Applied Bacteriology 43:1–24
    [Google Scholar]
  12. Hartl D. L., Dykhuizen D. E., Miller R. D., Green L., Framond J.DE. 1983; Transposable element IS50 improves growth rate of E. coli cells without transposition. Cell 35:503–510
    [Google Scholar]
  13. Herbert D., Phipps P. J., Tempest D. W. 1965; The chemostat: design and instrumentation. Laboratory Practice 14:1150–1161
    [Google Scholar]
  14. Höfle M. 1983; Long term changes in chemostat cultures of Cytophaga johnsonae . Applied and Environmental Microbiology 46:1045–1053
    [Google Scholar]
  15. Kyslik P., Sikyta B. 1984; Population changes in a culture of Escherichia coli K12 1EA growing in a ribitol-limited chemostat. Biotechnology Letters 6:25–30
    [Google Scholar]
  16. Monod J. 1950; La technique de culture continue; theorie et applications. Annales de l’Institut Pasteur 79:390–410
    [Google Scholar]
  17. Powell E. O. 1958; Criteria for the growth of contaminants and mutants in continuous culture. Journal of General Microbiology 18:259–268
    [Google Scholar]
  18. Schulze K. L., Lipe R. S. 1964; Relationship between substrate concentration, growth rate and respiration rate of Escherichia coli in continuous culture. Archiv für Mikrobiologie 48:1–20
    [Google Scholar]
  19. Tempest D. W., Neijssel O. M., Zevenboom W. 1983; Properties and performances of microorganisms in laboratory culture; their relevance to growth in natural ecosystems. Symposia of the Society for General Microbiology 34:119–152
    [Google Scholar]
  20. Westerhoff H. V. 1983 Mosaic non-equilibrium thermodynamics and (the control of) biological free- energy transduction Thesis University of Amsterdam;
    [Google Scholar]
  21. Westerhoff H. V., Lolkema J. S., Otto R., Hellingwerf K. J. 1982; Thermodynamics of growth. Non-equilibrium thermodynamics of bacterial growth. The phenomenological and the mosaic approach. Biochimica et biophysica acta 683:181–220
    [Google Scholar]
  22. Williamson J. R., Corkey B. E. 1969; Assays of intermediates of the citric acid cycle and related compounds by fluorometric enzyme methods. Methods in Enzymology 13:434–513
    [Google Scholar]
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