1887

Abstract

The locus of encodes an osmotically induced betaine transport system. We have identified a 31 kDa periplasmic protein, encoded by , whose synthesis is induced by osmotic stress. A specific betaine-binding activity with a of about 1 m is also present in the periplasm of osmotically induced cells. This activity is absent in those mutants which lack the 31 kDa periplasmic protein. Thus, ProU is a periplasmic binding-protein-dependent transport system.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-133-2-305
1987-02-01
2022-09-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/133/2/mic-133-2-305.html?itemId=/content/journal/micro/10.1099/00221287-133-2-305&mimeType=html&fmt=ahah

References

  1. Ames G.F.-L. 1974; Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Journal of Biological Chemistry 249:634–644
    [Google Scholar]
  2. Ames G.F.-L. 1986; Bacterial periplasmic transport systems: structure, mechanism, and evolution. Annual Review of Biochemistry 55:397–425
    [Google Scholar]
  3. Ames G.F.-L., Higgins C. F. 1983; Mechanisms, organization and evolution of periplasmic transport systems. Trends in Biochemical Science 8:97–100
    [Google Scholar]
  4. Berger E. A., Heppel L. A. 1974; Different mechanisms of energy coupling for the shock- sensitive and shock-resistant amino acid permeases of Escherichia coli . Journal of Biological Chemistry 249:7747–7755
    [Google Scholar]
  5. Bradford M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  6. Cairney J., Booth I. R., Higgins C. F. 1985a; Salmonella typhimurium proP gene encodes a transport system for the osmoprotectant betaine. Journal of Bacteriology 164:1218–1223
    [Google Scholar]
  7. Cairney J., Booth I. R., Higgins C. F. 1985b; Osmoregulation of gene expression in Salmonella typhimurium : proUencodes an osmotically induced betaine transport system. Journal of Bacteriology 164:1224–1232
    [Google Scholar]
  8. Clark D., Parker J. 1984; Proteins induced by high osmotic pressure in Escherichia coli . FEMS Microbiology Letters 25:81–83
    [Google Scholar]
  9. Dahl M. K., Manson M. D. 1985; Interspecific reconstitution of maltose transport and chemotaxis in Escherichia coliwith maltose-binding protein from various enteric bacteria. Journal of Bacteriology 164:1051–1063
    [Google Scholar]
  10. Dunlap V. J., Csonka L. N. 1985; Osmotic regulation of l-proline transport in Salmonella typhimurium . Journal of Bacteriology 163:296–304
    [Google Scholar]
  11. Gowrishankar J. 1985; Identification of osmore- sponsive genes in Escherichia coli: evidence for participation of potassium and proline transport systems in osmoregulation. Journal of Bacteriology 164:434–445
    [Google Scholar]
  12. Hall M. N., Silhavy T. J. 1981; The ompBlocus and the regulation of the major outer membrane porin proteins of Escherichia coli K12. Journal of Molecular Biology 146:23–43
    [Google Scholar]
  13. Hesse J. E., Wieczorek L., Altendorf K., Reicin A. S., Dorus E., Epstein W. 1984; Sequence homology between two membrane transport ATPases, the Kdp-ATPase of Escherichia coliand the Ca2+-ATPase of sarcoplasmic reticulum. Proceedings of the National Academy of Sciences of the United States of America 81:4746–4750
    [Google Scholar]
  14. Higgins C. F., Hardie M. M. 1983; Periplasmic protein associated with the oligopeptide permeases of Salmonella typhimuriumand Escherichia coli . Journal of Bacteriology 155:1434–1438
    [Google Scholar]
  15. Higgins C. F., Hiles I. D., Whalley K., Jamieson D. J. 1985; Nucleotide binding by membrane compounds of bacterial periplasmic binding protein-dependent transport systems. EMBO Journal 4:1033–1040
    [Google Scholar]
  16. Hiles I. D., Higgins C. F. 1986; Peptide uptake by Salmonella typhimurium:the periplasmic oligopeptide-binding protein. European Journal of Biochemistry 158:561–567
    [Google Scholar]
  17. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  18. Laimins L. A., Rhoads D. B., Epstein W. 1981; Osmotic control of kdp operon expression in Escherichia coli . Proceedings of the National Academy of Sciences of the United States of America 78:464–468
    [Google Scholar]
  19. Le Rudulier D., Valentine R. C. 1982; Genetic engineering in agriculture: osmoregulation. Trends in Biochemical Science 7:431–433
    [Google Scholar]
  20. Le Rudulier D., Strom A. R., Dandekar A. M., Smith L. T., Valentine R. C. 1984; Molecular biology of osmoregulation. Science 224:1064–1068
    [Google Scholar]
  21. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Perroud D., Le Rudulier D. 1985; Glycine betaine transport in Escherichia coli: osmotic modulation. Journal of Bacteriology 161:393–401
    [Google Scholar]
  23. Sutherland L., Cairney J., Elmore M. J., Booth I. R., Higgins C. F. 1986; Osmotic regulation of transcription: induction of the proUbetaine transport locus is determined by the accumulation of intracellular potassium. Journal of Bacteriology 168:805–814
    [Google Scholar]
  24. Yancey P. H., Clark M. E., Hand S. C., Bowlus R. D., Somero G. N. 1982; Living with water stress: evolution of osmolyte systems. Science 217:1214–1222
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-2-305
Loading
/content/journal/micro/10.1099/00221287-133-2-305
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error