SUMMARY: showed an absolute requirement for vitamin B for growth. Increase of cell number in vitamin B-deficient cultures occurred on the addition of vitamin B and depended on the amount added. The phosphate esters of vitamin B also supported growth. cells exhaustively took up vitamin B within about 2 h of addition. Of the total amount of vitamin B taken up, as much as 96% existed in the free form irrespective of the forms added. The addition of 4-amino-5-hydroxymethyl-2-methylpyrimidine, but not 5-(2-hydroxyethyl)-4-methylthiazole, to the vitamin B-deficient cells enhanced cell number at the same rate as the addition of vitamin B, indicating that Z is unable to synthesize the pyrimidine moiety of vitamin B.


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