1887

Abstract

SUMMARY: A technique is described for mathematically normalizing whole-cell protein profiles after sodium dodecyl sulphate-polyacrylamide gel electrophoresis to obtain standardized absolute migration distances using two internal standards. A soft laser scanning densitometer was used to measure protein band migration distances in wet, silver-stained gels. The normalized values were superior to the unnormalized migration distances and common values in reducing the inter- and intragel variability of the protein band positions. A procedure is described for clustering normalized bacterial protein profiles using a sample data set obtained from the type strains of four species.

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/content/journal/micro/10.1099/00221287-132-9-2653
1986-09-01
2019-10-14
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-9-2653
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