%0 Journal Article %A Plikaytis, Brian D. %A Carlone, George M. %A Plikaytis, Bonnie B. %T Numerical Analysis of Normalized Whole-cell Protein Profiles after Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis %D 1986 %J Microbiology, %V 132 %N 9 %P 2653-2660 %@ 1465-2080 %R https://doi.org/10.1099/00221287-132-9-2653 %I Microbiology Society, %X SUMMARY: A technique is described for mathematically normalizing whole-cell protein profiles after sodium dodecyl sulphate-polyacrylamide gel electrophoresis to obtain standardized absolute migration distances using two internal M r standards. A soft laser scanning densitometer was used to measure protein band migration distances in wet, silver-stained gels. The normalized values were superior to the unnormalized migration distances and common R F values in reducing the inter and intragel variability of the protein band positions. A procedure is described for clustering normalized bacterial protein profiles using a sample data set obtained from the type strains of four Legionella species. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-132-9-2653