@article{mbs:/content/journal/micro/10.1099/00221287-132-9-2653, author = "Plikaytis, Brian D. and Carlone, George M. and Plikaytis, Bonnie B.", title = "Numerical Analysis of Normalized Whole-cell Protein Profiles after Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis", journal= "Microbiology", year = "1986", volume = "132", number = "9", pages = "2653-2660", doi = "https://doi.org/10.1099/00221287-132-9-2653", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-132-9-2653", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: A technique is described for mathematically normalizing whole-cell protein profiles after sodium dodecyl sulphate-polyacrylamide gel electrophoresis to obtain standardized absolute migration distances using two internal M r standards. A soft laser scanning densitometer was used to measure protein band migration distances in wet, silver-stained gels. The normalized values were superior to the unnormalized migration distances and common R F values in reducing the inter and intragel variability of the protein band positions. A procedure is described for clustering normalized bacterial protein profiles using a sample data set obtained from the type strains of four Legionella species.", }