1887

Abstract

Summary: It is well established that the mitochondrial and the microsomal cytochromes in are regulated differently. Mutations affecting the mitochondrial cytochromes or had no effect on the concentration of the microsomal cytochrome P450 even during haem limitation. Moreover, a defect in the cytochrome P450 gene did not affect mitochondrial cytochromes. However, a regulatory mutation present in strain SG1 decreased both mitochondrial and microsomal cytochrome contents. This mutation also affected the intracellular haem concentration. The haem precursor 5-aminolaevulinate increased both mitochondrial and microsomal cytochrome contents. Our results indicate that carbon source and haem concentration are involved in the regulation of cytochrome P450.

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1986-08-01
2021-05-15
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References

  1. Aoyama Y., Yoshida Y., Hata S., Nishino T., Katsuki H., Maitra U. S., Mohan V. P., Sprinson D. B. 1983; Altered cytochrome P-450in a yeast mutant blocked in demethylating C-32 of lanosterol. Journal of Biological Chemistry 258:9040–9042
    [Google Scholar]
  2. Charalampous F. C., Chen W. 1974; Anaerobic synthesis of apocytochrome oxidase and assembly of the holoenzyme in yeast protoplasts. Journal of Biological Chemistry 249:1007–1013
    [Google Scholar]
  3. Fuhrhop J.-H., Smith K. M. 1975; Laboratory methods. In Porphyrins and Metalloporphyrins pp. 757–862 Edited by Smith K. M. Amsterdam: Elsevier;
    [Google Scholar]
  4. Gasser R., Hauri H.P., Meyer U. A. 1982; The turnover of cytochrome P450. FEBS Letters 147:239–242
    [Google Scholar]
  5. Gollub E.G., Trocha P., Liu P. K., Sprinson D. B. 1974; Yeast mutants requiring ergosterol as only lipid supplement. Biochemical and Biophysical Research Communications 56:471–477
    [Google Scholar]
  6. Gollub E.G., Liu K. P., Dayan J., Adlersberg M., Sprinson D. B. 1977; Yeast mutants deficient in heme biosynthesis and a heme mutant additionally blocked in cyclization of 2,3-oxidosqualene. Journal of Biological Chemistry 252:2846–2854
    [Google Scholar]
  7. Guarente L., Mason TH. 1983; Heme regulates transcription of the CYCI gene of S. cerevisiae via an upstream regulation site. Cell 32:1279–1286
    [Google Scholar]
  8. Guarente L., Lalonde B., Gifford P., Alani E. 1984; Distinctly regulated tandemupstream activation sites mediate catabolite repression of the CYCI gene of S. cerevisiae. Cell 36:503–511
    [Google Scholar]
  9. Haslam J. M., Astin A. M. 1979; The use of hemedeficient mutants to investigate mitochondrial function and biogenesis in yeast. Methods in Enzymology 56:558–568
    [Google Scholar]
  10. Hoertner H., Ammerer G., Hartter E., Hamilton B., Rytka J., Bilinski T., Ruis H. 1982; Regulation of synthesis of catalases and iso-lcytochrome c in Saccharomyces cerevisiae by glucose, oxygen and heme. European Journal of Biochemistry 128:179–184
    [Google Scholar]
  11. Ishidate K., Kawaguchi K., Tagawa K., Hagihara B. 1969a; Hemoproteins in anaerobically grown yeast cells. Journal of Biochemistry 65:375–383
    [Google Scholar]
  12. Ishidate K., Kawaguchi K., Tagawa K. 1969b; Change in cytochrome content accompanying aerobic formation of mitochondria in yeast. Journal of Biochemistry 65:385–392
    [Google Scholar]
  13. Kaeppeli O., Sauer M., Fiechter A. 1982; Convenient procedure for the isolation of highly enriched cytochrome P450-containing microsomal fraction from Candida tropicalis. Analytical Biochemistry 126:179–182
    [Google Scholar]
  14. Labbe-Bois R., Volland CH. 1977; Changes in the activities of the protoheme-synthesizing system during the growth of yeast under different conditions. Archives of Biochemistry and Biophysics 179:565–577
    [Google Scholar]
  15. Mahler H. R., Lin C.-C. 1978; Molecular events during the release of δ-aminolevulinate dehydratase from catabolite repression. Journal of Bacteriology 135:54–61
    [Google Scholar]
  16. Matner R. R., Sherman F. 1982; Differential accumulation of two apo-isocytochromes c in processing mutants of yeast. Journal of Biological Chemistry 257:9811–9821
    [Google Scholar]
  17. Sanders H. K., Mied P.A., Briquet M., Hernandez-Rodriguez J., Gottal R. F., Mattoon J. R. 1973; Regulation of mitochondrial biogenesis: yeast mutants deficient in synthesis of deltaaminolevulinic acid. Journal ofMolecular Biology 80:17–39
    [Google Scholar]
  18. Sanglard D., Kaeppeli O., Fiechter A. 1984; Metabolic conditions determining the composition and catalytic activity ofcytochrome P-450 monooxygenase in Candida tropicalis. Journal of Bacteriology 157:297–302
    [Google Scholar]
  19. Schatz G., Butlow A. 1983; How are proteins imported into mitochondria?. Cell 32:316–318
    [Google Scholar]
  20. Sherman F., Steward J. 1971; Genetics and biosynthesis of cytochrome c. Annual Redew of Genetics 5:257–296
    [Google Scholar]
  21. Sherman F., Fink G. R., Lawrence C. W. (eds) 1974; Methods in Yeast Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  22. Trocha P. J., Jasne S. J., Sprinson D. B. 1977; Yeast mutants blocked in removing the methyl group of lanosterol at C-14. Separation of sterols by high pressure liquid chromatography. Biochemistry 16:4721–4726
    [Google Scholar]
  23. Wiseman A., Lim T. K., Mccloud C. 1975; Relationship of cytochrome P450 to growth phase of Brewer’s yeast in 1% or 20% glucose medium. Biochemical Society Transactions 5:276–278
    [Google Scholar]
  24. Woods R.A., Sanders H. K., Briquet M., Foury F., Drysdale B.-E., Mattoon J. R. 1975; Regulation of mitochondrial biogenesis: enzymatic changes in cytochrome-deficient yeast mutants requiring aminolevulinic acid. Journal of Biological Chemistry 250:9090–9098
    [Google Scholar]
  25. Yoshida Y., Kumaoka H., Sato R. 1974; Studies on the microsomal electron-transport system of anaerobically grown yeast. Journal of Biochemistry 75:1210
    [Google Scholar]
  26. Yoshinaga T., Sassa SH., Kappas A. 1982; A comparative study of heme degradation by NADPH-cytochrome c reductase alone and by its complete heme oxygenase system. Journal of Biological Chemistry 257:7794–7802
    [Google Scholar]
  27. Zitomer R. S., Montgomery D. L., Nichols D. L., Hall B. D. 1979; Transcriptional regulation of the yeast cytochrome c gene. Proceedings of the National Academy of Sciences of the United States of America 76:3627–3631
    [Google Scholar]
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