1887

Abstract

A wide range of nitriles and amides act as carbon and/or nitrogen sources for LL100-21. Growth on a number of aliphatic nitriles or their corresponding amides resulted in the coinduction of both acetonitrile hydratase and acetamidase activities. In each case the specific activity of acetonitrile hydratase was higher than that of acetamidase. The substrate and inducer specificities were determined for the two enzymes and found to be quite distinct. Propionitrile, although an excellent inducer of both enzymes, was only slowly hydrolysed by acetonitrile hydratase. Conversely acrylonitrile was a good enzyme substrate but had a complete lack of effect as an inducer. Butyramide and methacrylamide acted as poor substrates for acetamidase but induced high levels of both acetonitrile hydratase and acetamidase. Dinitriles of long chain length, lactonitrile and iodoacetamide were inhibitory to growth and enzyme activity.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-6-1493
1986-06-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/6/mic-132-6-1493.html?itemId=/content/journal/micro/10.1099/00221287-132-6-1493&mimeType=html&fmt=ahah

References

  1. Arnaud A., Galzy P., Jallageas J.-C. 1976a; Amidase activity of some bacteria. . Folia micro- biologica (Praha) 21:178–184
    [Google Scholar]
  2. ARNAUD A., GALZY P., JALLAGEAS J.-C. 1976b; Etude de l’activite nitrilasique de quelques bacte- ries. . Revue des fermentations et des industries alimen- taires 31:39–44
    [Google Scholar]
  3. ARNAUD A., GALZY P., JALLAGEAS J.-C. 1977; Etude de l’acetonitrilase d’une souche de Brevibacte- rium. . Agricultural and Biological Chemistry 41:2183–2191
    [Google Scholar]
  4. ASANO Y., TANI Y., YAMADA H. 1980; A new enzyme ‘nitrile hydratase’ which degrades aceto- nitrile in combination with amidase.. Agricultural and Biological Chemistry 44:2251–2252
    [Google Scholar]
  5. ASANO Y., FUJISHIRO K., TANI Y., YAMADA H. 1982; Aliphatic nitrile hydratase from Arthrobacter sp. J-l. Purification and characterisation.. Agricultural and Biological Chemistry 46:1165–1174
    [Google Scholar]
  6. BAUCHOP T., ELSDEN S. R. 1960; The growth of micro-organisms in relation to their energy supply.. Journal of General Microbiology 23:457–469
    [Google Scholar]
  7. CLARKE P. H. 1970; The aliphatic amidases of Pseudomonas aeruginosa. . Advances in Microbial Physiology 4:179–222
    [Google Scholar]
  8. CLARKE P. H. 1980; The utilization of amides by microorganisms.. In Microorganisms and Nitrogen Sources pp. 537–562 editor Payne J. W. . New York: John Wiley & Sons;
    [Google Scholar]
  9. COLLINS P. A., KNOWLES C. J. 1983; The utilization of nitriles and amides by Nocardia rhodochrous.. Journal of General Microbiology 129:711–718
    [Google Scholar]
  10. DIGERONIMO M. J., ANTOINE A. D. 1976; Metabolism of acetonitrile and propionitrile by Nocardia rhodochrous LL100 21. Applied and Environmental Microbiology 31:900–906
    [Google Scholar]
  11. FAWCETT J. K., SCOTT J. E. 1960; A rapid and precise method for the determination of urea.. Journal of Clinical Pathology 13:156–159
    [Google Scholar]
  12. GORNALL A. G., BARDAWILL C. J., DAVID M. M. 1949; Determination of serum proteins by the biuret reaction. . Journal of Biological Chemistry 177:751–766
    [Google Scholar]
  13. HARPER D. B. 1976; Purification and properties of an unusual nitrilase from Nocardia NCIB 11216. Biochemical Society Transactions 4:502–504
    [Google Scholar]
  14. HARPER D. B. 1977a; Microbial metabolism of aromatic nitriles. Enzymology of C-N cleavage by Nocardia sp. (Rhodochrous group) NCIB 11216. Biochemical Journal 165:309–319
    [Google Scholar]
  15. HARPER D. B. 1977b; Fungal degradation of aromatic nitriles. Enzymology of C-N cleavage by Fusar- ium solani. Biochemical Journal 167:685–692
    [Google Scholar]
  16. HOOK R. H., ROBINSON W. G. 1964; Ricinine nitrilase. II. Purification and properties. . Journal of Biological Chemistry 239:4263–4267
    [Google Scholar]
  17. JALLAGEAS J., -C., ARNAUD A., GALZY P. 1978; Etude l’acetamidase d’une souche de Brevibacterium. . Journal of General and Applied Microbiology 24:103–114
    [Google Scholar]
  18. MILLER J. H. 1972; Experiments in Molecular Genetics, p. . 431 Cold Spring Harbor: NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  19. Robinson W. G., Hook R. H. 1964; Ricinine nitrilase. I. Reaction product and substrate specificity. . Journal of Biological Chemistry 239:4257–4262
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-6-1493
Loading
/content/journal/micro/10.1099/00221287-132-6-1493
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error