1887

Abstract

Summary: A radiorespirometric study of glucose metabolism in CBS 621 was done using glucose-limited chemostat cultures growing at a dilution rate of 0.1 h with ammonium or nitrate as the nitrogen source. From a steady-state analysis of CO yields from [1-C]- and [6-C]glucose supplied in the medium feed it appeared that during growth with nitrate the flow of glucose through the hexose monophosphate (HMP) pathway was much higher than during growth with ammonium as the nitrogen source. The same phenomenon was apparent from an analysis of the rate of CO production after administration of small amounts of labelled glucose to samples withdrawn from steady-state cultures. Additionally, these experiments revealed not only that the initial fraction of glucose 6-phosphate routed into the HMP pathway increases, but also that recycling of hexose phosphates via this pathway increases when nitrate is used as the nitrogen source. From a quantitative analysis of the results it is concluded that the contribution of the HMP pathway to glucose metabolism is close to the theoretical minimum required to cover the NADPH requirement for biosynthesis.

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/content/journal/micro/10.1099/00221287-132-2-221
1986-02-01
2024-12-07
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References

  1. Bruinenberg P. M., van Dijken J. P., Scheffers W. A. 1983a; A theoretical analysis of NADPH production and consumption in yeasts. Journal of General Microbiology 129:953–964
    [Google Scholar]
  2. Bruinenberg P. M., van Dijken J. P., Scheffers W. A. 1983b; An enzymic analysis of NADPH production and consumption in Candida utilis CBS 621. Journal of General Microbiology 129:965–971
    [Google Scholar]
  3. Bruinenberg P. M., Jonker R., van Dijken J. P., Scheffers W. A. 1985a; Utilization of formate as an additional energy source by glucose-limited chemostat cultures of Candida utilis CBS 621 and Saccharomyces cerevisiae CBS 8066. Evidence for the absence of transhydrogenase activity in yeasts. . Archives of Microbiology 142:302–306
    [Google Scholar]
  4. Bruinenberg P. M., van Dijken J. P., Kuenen J. G., Scheffers W. A. 1985b; Oxidation of NADH and NADPH by mitochondria from the yeast Candida utilis. Journal of General Microbiology 131:1043–1051
    [Google Scholar]
  5. Dawson P. S. S., Okada W., Steinhauer L. P. 1976; Some comparative observations on the relative contributions of alternate pathways in the metabolism of glucose by Candida utilis. Canadian Journal of Microbiology 22:996–1001
    [Google Scholar]
  6. Den Hollander J. A., Brown T. R., Ugurbil K., Shulman R. G. 1979; 13C nuclear magnetic resonance studies of anaerobic glycolysis in suspensions of yeast cells. Proceedings of the National Academy of Sciences of the United States of America 76:6096–6100
    [Google Scholar]
  7. Gancedo J. M., Lagunas R. 1973; Contribution of the pentose-phosphate pathway to glucose metabolism in Saccharomyces cerevisiae: a critical analysis on the use of labelled glucose. Plant Science Letters 1:193–200
    [Google Scholar]
  8. Harder W., Visser K., Kufnen J. G. 1974; Laboratory fermenter with an improved magnetic drive. Laboratory Practice 23:644–645
    [Google Scholar]
  9. Katz J., Rognstad R. 1967; The labeling of pentose phosphate from glucose-14C and estimation of the rates of transaldolase, transketolase, the contribution of the pentose cycle, and ribose phosphate synthesis. Biochemistry 6:2227–2247
    [Google Scholar]
  10. Katz J., Wood H. G. 1963; The use of C’4O2 yields from glucose-1 and -6-C14 for the evaluation of the pathways of glucose metabolism. Journal of Biological Chemistry 238:517–523
    [Google Scholar]
  11. Mian F. A., Fencl Z., Prokop A., Mohagheghi A., Fazeli A. 1974; Effect of growth rate on the glucose metabolism of yeast grown in continuous culture. Radiorespirometric studies. Folia microbio-logica 19:191–198
    [Google Scholar]
  12. Robertson L. A., Kuenen J. G. 1984; Aerobic denitrification - old wine in new bottles?. Antonie van Leeuwenhoek 50:525–544
    [Google Scholar]
  13. Verduyn C., van Dijken J. P., Scheffers W. A. 1984; Colorimetric alcohol assays with alcohol oxidase Journal of Microbiological Methods. Microbiological Methods 2:15–25
    [Google Scholar]
  14. Wang C. H. 1972; Radiorespirometric methods. Methods in Microbiology 6B:185–230
    [Google Scholar]
  15. Wang C. H., Krackov J. K. 1962; The catabolic fate of glucose in Bacillus subtilis. Journal of Biological Chemistry 237:3614–3622
    [Google Scholar]
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