1887

Abstract

SUMMARY: A non-inducing medium (NID) was defined for studying the induction of methanethiol production by CNRZ 918. The lowest -methionine concentration capable of inducing maximal methanethiol production by the cells was 7 m. The peptides -Ala--Met and -Met--Ala induced greater methanethiol production than free -methionine. -Methionine, -cysteine, -methyl--cysteine and -ethionine were poor inducers. Culture temperature affected the duration of induction. An Na concentration of 1 in the culture medium led to maximal methanethiol production capacity of both cells and cell extracts. -Methionine and -ethionine were the best substrates for the crude soluble cells extract (with release of methanethiol and ethanethiol respectively). Neither the derivatives of -methionine that acted as inducers, nor -methionine, were substrates for demethiolation. Demethiolation activity of the crude extract was thermolabile, not stimulated by Na and strongly inhibited by Zn, Mn and Cu. The shortest generation time obtained for CNRZ 918 in NID medium + -methionine was 4 h at 26 °C. Only coccoid forms were present when the culture temperature was 30 °C. The presence of -methionine in the medium favoured their appearance. The strain grew best in the presence of 1% NaCl but tolerated concentrations up to 15%. The induction of methanethiol production was due to the induction of -methionine-γ-demethiolase. The level of induction was probably related to the intracellular concentration of -methionine. The transport system of -methionine by CNRZ 918 was constitutive and Na dependent.

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1986-11-01
2021-10-28
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References

  1. Esaki N., Suzuki T., Tanaka H., Soda K., Rando R. R. 1977; Deamination and γ-addition reactions of vinyl-glycine by l-methionine-γ-lyase. FEBS Letters 84:309–312
    [Google Scholar]
  2. Ferchichi M., Hemme D., Nardi M., Pamboukdjian N. 1985; Production of methanethiol from methionine by Brevibacterium linens CNRZ 918. Journal of General Microbiology 131:715–723
    [Google Scholar]
  3. Ito S., Nakamura T., Eguchi Y. 1976; Purification and characterization of methioninase from Pseudomonas putida. Journal of Biochemistry 79:1263–1272
    [Google Scholar]
  4. Kadota H., Ishida Y. 1972; Production of volatile sulfur compounds by microorganisms. Annual Review of Microbiology 26:127–138
    [Google Scholar]
  5. Kallio R. E., Larson A. D. 1955; Methionine degradation by species of Pseudomonas. In Sympo¬sium on Amino Acid Metabolism pp. 616–634 Edited by McElray W. D., Glass H. B. Baltimore: Johns Hopkins Press;
    [Google Scholar]
  6. Kreiss W., Hession C. 1973; Isolation and purification of l-methionine α-deamino-γ-mercapto-methane-lyase (l-methioninase) from Clostridium sporogenes. Cancer Research 33:1862–1865
    [Google Scholar]
  7. Laakso S. 1979; Evidence of multiple demethiolation of methionine by a methionine utilizing mutant of Pseudomonas fluorescens UK1. FEMS Microbiology Letters 5:407–409
    [Google Scholar]
  8. Laakso S., Laukkanen N. 1982; The role of 2-aminobutyrate in methionine utilization by Pseudomonas fluorescens UK1. FEMS Microbiology Letters 14:107–111
    [Google Scholar]
  9. Laakso S., Nurmikko V. 1976; A spectrophoto-metric assay for demethiolating activity. Analytical Biochemistry 72:600–605
    [Google Scholar]
  10. Laakso S., Soderling E., Nurmikko V. 1976; Methionine degradation by Pseudomonas fluorescens UK1 and its methionine utilizing mutant. Journal of General Microbiology 94:305–312
    [Google Scholar]
  11. Law B. A. 1984; Flavour development in cheeses. In Advances in the Microbiology and Biochemistry of Cheese and Fermented Milk pp. 187–208 Edited by Davies F. L., Law B. A. London New York: Elsevier Applied Science Publishers;
    [Google Scholar]
  12. Law B. A., Sharpe M. E. 1978; Formation of methanethiol by bacteria isolated from raw milk and Cheddar cheese. Journal of Dairy Research 45:267–275
    [Google Scholar]
  13. Merricks D. L., Salsbury R. L. 1975; Dethiomethylation of methionine by an extract of rumen protozoa: general substrate specificity. Journal of Animal Science 42:955–959
    [Google Scholar]
  14. Nakayama T., Esaki N., Lee W. J., Tanaka I., Tanaka H., Soda K. 1984a; Purification and properties of l-methionine-γ-lyase from Aeromonas sp. Agricultural and Biological Chemistry 9:2367–2369
    [Google Scholar]
  15. Nakayama T., Esaki N., Sugie K., Beresov T. T., Tanaka H., Soda K. 1984b; Purification of l-methionine-γ-lyase. Analytical Biochemistry 138:421–424
    [Google Scholar]
  16. Rice G., Stewart F. H. C., Hillier A. J., Jago G. R. 1978; The uptake of amino-acids and peptides by Streptococcus lactis. Journal of Dairy Research 45:93–107
    [Google Scholar]
  17. Richard J. 1984; Evolution de la flore microbienne a la surface des Camemberts fabriques avec du lait cru. Le Lait 64:496–520
    [Google Scholar]
  18. Ruiz-Herrera J., Starkey R. L. 1969; Dissimilation of methionine by demethiolase of Aspergillus species. Journal of Bacteriology 99:766–770
    [Google Scholar]
  19. Ruiz-Herrera J., Starkey R. L. 1970; Dissimilation of methionine by Achromobacter starkeyi. Journal of Bacteriology 104:1286–1293
    [Google Scholar]
  20. Sharpe M. E., Law B. A., Philipps B. A., Pitcher D. G. 1977; Methanethiol production by coryneform bacteria: strains from dairy and human skin sources and Brevibacterium linens. Journal of General Microbiology 101:345–349
    [Google Scholar]
  21. Shiio I., Miyajima R., Kashima N. 1973; Na+ dependent transport of threonine in Brevibacterium flavum. Journal of Biochemistry 73:1185–1193
    [Google Scholar]
  22. Tanaka H., Esaki N., Yamamoto T., Soda K. 1976; Purification and properties of methioninase from Pseudomonas ovalis. FEBS Letters 66:307–311
    [Google Scholar]
  23. Tanaka H., Esaki N., Soda K. 1977; Properties of l-methionine-γ-lyase from Pseudomonas ovalis. Biochemistry 16:100–106
    [Google Scholar]
  24. Tanaka H., Esaki N., Soda K. 1985; A versatile bacterial enzyme: l-methionine-γ-lyase. Enzyme and Microbial Technology 7:530–537
    [Google Scholar]
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