1887

Abstract

Summary: A new mutation, D, determining resistance of to nalidixic acid (NAL) is reported. The mutant described is resistant to NAL at 37°C but sensitive at 30 C. It is defective in penetration of NAL and glycerol through the outer membrane at 37°C. The mutation is located half-way between 89 and 89.5 min on the genetic map.

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/content/journal/micro/10.1099/00221287-131-9-2285
1985-09-01
2021-05-17
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References

  1. Bachmann B. 1983; Linkage map of Escherichia coli K-12 Edition 7. Microbiological Reviews 47:180–230
    [Google Scholar]
  2. Bourguignon G. J., Levitt M., Sternglanz R. 1973; Studies on the mechanism of action of nalidixic acid. Antimicrobial agents and Chemotherapy 4:479–486
    [Google Scholar]
  3. Cozzarellni R. 1980; DNA gyrase and the supercoiling DNA. Science 207:953–960
    [Google Scholar]
  4. Davis B. D., Mingioli E. S. 1965; Mutants of Escherichia coli requiring methionine or vitamin B12 . Journal of Bacteriology 60:17–19
    [Google Scholar]
  5. Decad G., Nikaido H. 1976; Outer membrane of Gram-negative bacteria. XII. Molecular-sieving function of cell wall. Journal of Bacteriology 128:225–236
    [Google Scholar]
  6. Engle E. C., Manes S. H., Drlica K. 1982; Differential effects of antibiotics inhibiting gyrase. Journal of Bacteriology 149:92–98
    [Google Scholar]
  7. Goss W. A., Deitz W. H., Cook T. M. 1965; Mechanism of action of nalidixic acid on Escherichia coli. II.Inhibition of deoxyribonucleic acid synthesis. Journal of Bacteriology 89:1068–1074
    [Google Scholar]
  8. Hane M., Wood T. 1969; Escherichia coli K-12 mutants resistant to nalidixic acid: genetic mapping and dominance studies. Journal of Bacteriology 99:238–241
    [Google Scholar]
  9. Howard-Flanders P., Boyce R. P., Theriot L. 1966; Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics 53:1119–1139
    [Google Scholar]
  10. Inouye S., Ohne T., Yamagis J., Nakamura S., Shimizu M. 1978; Mode of incomplete cross-resistance among pipemidic, piromidic and nalidixic acids. Antimicrobial Agents and Chemotherapy 14:240–245
    [Google Scholar]
  11. Kumar S. 1980; Types of spontaneous nalidixic acid mutants of Escherichia coliM . Indian Journal of Experimental Biology 18:341–343
    [Google Scholar]
  12. Kuwano M., Taniguchi H., Ono M., Endo H., Ohnishi Y. 1977; An Escherichia coli K-12 mutant carrying altered ribosomal protein S10. Biochemical and Biophysical Research Communications 15:156–162
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavageofstructural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. Levinthal C., Singer E. R., Fetharolf K. 1962; Reactivation and hybridization of reduced alkaline phosphatase. Proceedings of the National Academy of Sciences of the United States of America 48:1230–1232
    [Google Scholar]
  15. Lin E.C.C. 1976; Glycerol dissimilation and its regulation in bacteria. Annual Review of Microbiology 30:535–578
    [Google Scholar]
  16. Low B. 1973; Rapid mapping of conditional mutations in Escherichia coli K-12. Journal of Bacteriology 113:798–822
    [Google Scholar]
  17. Lugtenberg B., Meijers J., Peters R., Van Der Hoek P., Vana Lphen L. 1975; Electrophoretic resolution of the major outer membrane protein of Escherichia coli K-12 into four bands. FEBS Letters 58:254–258
    [Google Scholar]
  18. Miller J. H. 1972 Experiments in Molecular Genetics. Cold Spring Harbor New York:: Cold Spring Harbor Laboratory;
    [Google Scholar]
  19. Nakae T. 1976; Identification of the outer membrane protein of Escherichia coli that produces transmembrane channls. Biochemical and Biophysical Research Communications 71:877–884
    [Google Scholar]
  20. Nakae Ishii J. 1980; Permeability propertiesof Escherichia coli outer membrane containing poreformingproteins : comparison between lambda, receptor protein and porin for saccharide permpermeation. Journal of Bacteriology 142:735–740
    [Google Scholar]
  21. Ono M., Kuwano M. 1978; Mutation affecting the thermolability of the 50S ribosomal subunitEscherichia coli . Journal of Bacteriol 134:677–679
    [Google Scholar]
  22. Ozawa Y., Mizushim S. 1983; Regulation of outer membrane porin protein synthesis in Escherichia coli K-12, ompF regulates the expression of ompC. Journal of Bacteriology 154:669–675
    [Google Scholar]
  23. Pedrini A. M. 1979; Nalidixic acid, mechanism of action of antibacterial agents. In Antibiotics V part 1 pp. 154–175 Edited by Hahn F. E., Berlin Heidelberg. New York: Springer-Verlag
    [Google Scholar]
  24. Pluciennik H., Hrebenda J. 1982; Labelling of nalidixic acid with tritium. Radiochemical and Radioanalytical Letters 56:25–34
    [Google Scholar]
  25. Scudamore R. A., Beveridge T. J., Goldmer M. 1979; Outer-membrane penetration barriers as components of intrinsic resistance to beta-lactam and other antibiotics in Escherichia coli K-12. Antimic-robial Agents and Chemotherapy 15:182–189
    [Google Scholar]
  26. Sugino A., Peebles C.L., Kreuzer K. N., Cozzarelli N. R. 1977; Mechanisms of action of nalidixic acid. Purification of Escherichia coli nalAgene product and its relationship to DNA gyrase, a novel nicking-closing enzyme. Proceedings of the National Academy of Sciences of the United States America 74:4767–4771
    [Google Scholar]
  27. Szmelcman S., Schwartz M., Silhavy T.J., Boos W. 1976; Maltose transport in Escherichia coli K-12 A comparison of transport kinetics in wild-type and λ-resistant mutants with the dissociation constants of the maltose-binding protein as measured by fluorescence quenching. European Journal of Biochemistry 65:13–19
    [Google Scholar]
  28. Van Alphen W., Lugtenberg B. 1977; Influence of osmolarity of the growth medium on the outer membrane protein pattern of Escherichia coli . Journal nal ll Bacteriology 131:623–630
    [Google Scholar]
  29. Watson R. J., Parker J., Fill N. P., Flaks J. G., Friesen J.D. 1975; New chromosomal location for structural genes of ribosomal proteins. Proceedings of the National Academy of Sciences of the United States of America 72:2765–2769
    [Google Scholar]
  30. Wu T. T.M. 1966; A model for three-point analysis of random general transduction. Genetics 54:405–410
    [Google Scholar]
  31. Yamaguchi A., Himura R., Sawai T. 1982; Phospholipid bilayer permeability of P-lactam antibiotics. Journal of Antibiotics 35:1692–1699
    [Google Scholar]
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