1887

Abstract

Summary: A mutant of has been constructed with deletions of the genes encoding penicillin-binding protein 5 () and penicillin-binding protein 6 (). The construction of this mutant establishes that the complete loss of the two most abundant species of penicillin-binding proteins can be tolerated by . Moreover, the double deletion mutant had the same growth rate and morphology as an isogenic strain.

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/content/journal/micro/10.1099/00221287-131-8-2115
1985-08-01
2024-05-05
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References

  1. Amanuma H., Strominger J. L. 1980; Purification and properties of penicillin-binding proteins 5 and 6 from Escherichia coli membranes. Journal of Biological Chemistry 255:11171–11180
    [Google Scholar]
  2. Broome-Smith J. K., Spratt B. G. 1982; Deletion of the penicillin-binding protein 6 gene of Escherichia coli . Journal of Bacteriology 152:904–906
    [Google Scholar]
  3. Broome-Smith J., Spratt B. G. 1984; An amino acid substitution that blocks the deacylation step in the enzyme mechanism of penicillin-binding protein 5 of Escherichia coli . FEBS Letters 165:185–189
    [Google Scholar]
  4. Broome-Smith J. K., Edelman A., Spratt B. G. 1983; Sequence of penicillin-binding protein 5 of Escherichia coli . In The Target of Penicillin pp 403–408 Berlin: de Gruyter & Co;
    [Google Scholar]
  5. Glauner B., Keck W., Schwarz U. 1984; Analysis of penicillin-binding sites with a micro method – the binding site of PBP 5 from Escherichia coli . FEMS Microbiology Letters 23:151–155
    [Google Scholar]
  6. Iwaya M., Strominger J. L. 1977; Simultaneous deletion of d-alanine carboxypeptidase 1B-C activity and penicillin-binding component IV of Escherichia coli K12. Proceedings of the National Academy of Sciences of the United States of America 74:2980–2984
    [Google Scholar]
  7. Matsuhashi M., Nakagawa J., Tomioka S., Ishino F., Tamaki S. 1982; Mechanism of peptidoglycan synthesis by penicillin-binding proteins in bacteria and effects of antibiotics. In Drug Resistance in Bacteria – Genetics, Biochemistry and Molecular Biology pp 297–310 Edited by Mitsuhashi S. Tokyo: Japan Scientific Societies Press;
    [Google Scholar]
  8. McEntee K. 1976; Specialised transduction of recA by bacteriophage lambda. Virology 70:221–222
    [Google Scholar]
  9. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  10. Nakagawa J., Tamaka S., Matsuhashi M. 1979; Purified penicillin-binding proteins IBs from Escherichia coli membrane showing activities of both peptidoglycan polymerase and peptidoglycan crosslinking enzyme. Agricultural and Biological Chemistry 43:1379–1380
    [Google Scholar]
  11. Spratt B. G. 1975; Distinct penicillin-binding proteins involved in the division, elongation and shape of Escherichia coli K12. Proceedings of the National Academy of Sciences of the United States of America 72:2999–3003
    [Google Scholar]
  12. Spratt B. G. 1980; Deletion of the penicillin-binding protein 5 gene of Escherichia coli . Journal of Bacteriology 144:1190–1192
    [Google Scholar]
  13. Spratt B. G. 1983; Penicillin-binding proteins and the future of β-lactam antibiotics. Journal of General Microbiology 129:1247–1260
    [Google Scholar]
  14. Stoker N. G., Fairweather N. F., Spratt B. G. 1982; Versatile low-copy-number plasmid vectors for cloning in Escherichia coli . Gene 18:335–341
    [Google Scholar]
  15. Suzuki H., Van Heijenoort Y., Tamura T., Mizoguchi J., Hirota Y., Van Heijenoort J. 1980; In vitro peptidoglycan polymerisation catalysed by penicillin-binding protein 1B of Escherichia coli K12. FEBS Letters 110:245–249
    [Google Scholar]
  16. Tamura T., Imae Y., Strominger J. L. 1976; Purification to homogeneity and properties of two d-alanine carboxypeptidases 1 from Escherichia coli . Journal of Biological Chemistry 251:414–423
    [Google Scholar]
  17. Twigg A. J., Sherratt D. 1980; Trans-complementable copy-number mutants of plasmid Col E1. Nature, London 283:216–218
    [Google Scholar]
  18. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
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