1887

Abstract

Summary: Yeast cells of the temperature-sensitive morphological mutant strain Mc3 of the polymorphic fungus were allowed to initiate multicellular-form development at the restrictive temperature (37 °C), then induced to resume budding yeast growth by downshift to the permissive temperature (25 °C). The kinetics of the resumption of yeast budding were investigated, and the dependence of bud initiation upon DNA synthesis, protein synthesis and microtubule function was probed. The results indicated that the thermolabile event in strain Mc3 was a cell cycle event involved in yeast budding, and that developing multicellular forms retained most of the machinery necessary to reinitiate budding. However, initiation of budding required the synthesis of protein, and possibly RNA, suggesting that one or more proteins involved in initiation of budding were not present in the developing multicellular forms.

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/content/journal/micro/10.1099/00221287-131-7-1719
1985-07-01
2024-12-10
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References

  1. Cooper C. R., Harris J. L., Jacobs C. W., Szaniszlo P. J. 1984; Effects of polyoxin AL on cellular development in Wangiella dermatitidis . Experimental Mycology 8:349–363
    [Google Scholar]
  2. DeKloet S. R. 1966; The effect of cycloheximide on the synthesis of ribonucleic acid in Saccharomyces carlsbergensis . Biochemical Journal 99:566–581
    [Google Scholar]
  3. Elford H. L. 1968; Effect of hydroxyurea on ribonucleotide reductase. Biochemical and Biophysical Research Communications 33:129–135
    [Google Scholar]
  4. Fiala E. S., Davis F. F. 1965; Preferential inhibition of synthesis and methylation of ribosomal RNA in Neurospora crassa by Actidione. Biochemical and Biophysical Research Communications 18:115–118
    [Google Scholar]
  5. Fukuhara H. 1965; RNA synthesis in yeast in the presence of cycloheximide. Biochemical and Biophysical Research Communications 18:297–301
    [Google Scholar]
  6. Haidle C. W., Storck R. 1966; Inhibition by cycloheximide of protein and RNA synthesis in Mucor rouxii . Biochemical and Biophysical Research Communications 22:175–180
    [Google Scholar]
  7. Horgen P. A., Griffin D. H. 1971; Specific inhibitors of the three RNA polymerases from the aquatic fungus Blastocladiella emersonii . Proceedings of the National Academy of Sciences of the United States of America 68:338–341
    [Google Scholar]
  8. Jacobs C. W., Szaniszlo P. J. 1982; Microtubule function and its relation to cellular development and the yeast cell cycle in Wangiella dermatitidis . Archives of Microbiology 133:155–161
    [Google Scholar]
  9. Jacobs C. W., Szaniszlo P. J. 1985; Altered development in a temperature-sensitive morphological mutant of Wangiella dermatitidis . Mycologia 77:142–148
    [Google Scholar]
  10. McGinnis M. R. 1983; Chromomycosis and phaeo-hyphomycosis: new concepts, diagnosis, and mycology. Journal of the American Academy of Dermatology 8:1–16
    [Google Scholar]
  11. Nishimura K., Miyaji M. 1983; Defense mechanism of mice against Exophiala dermatitidis infection. Mycopathologia 81:9–21
    [Google Scholar]
  12. Oujezdsky K. B., Grove S. N., Szaniszlo P. J. 1973; Morphological and structural changes during yeast-to-mold conversion of Phialophora dermatitidis . Journal of Bacteriology 113:468–477
    [Google Scholar]
  13. Roberts R. L., Szaniszlo P. J. 1978; Temperature-sensitive multicellular mutants of Wangiella dermatitidis . Journal of Bacteriology 135:622–632
    [Google Scholar]
  14. Roberts R. L., Szaniszlo P. J. 1980; Yeast-phase cell cycle of Wangiella dermatitidis . Journal of Bacteriology 144:721–731
    [Google Scholar]
  15. Roberts R. L., Lo R. J., Szaniszlo P. J. 1979; Nuclear division in temperature-sensitive multicellular mutants of Wangiella dermatitidis . Journal of Bacteriology 137:1456–1458
    [Google Scholar]
  16. Roberts R. L., Lo R. J., Szaniszlo P. J. 1980; Induction of synchronous growth in the yeast phase of Wangiella dermatitidis . Journal of Bacteriology 141:981–984
    [Google Scholar]
  17. Szaniszlo P. J., Hseih P. H., Marlowe J. D. 1976; Induction and ultrastructure of the multicellular (sclerotic) morphology in Phialophora dermatitidis . Mycologia 68:117–130
    [Google Scholar]
  18. Szaniszlo P. J., Geis P. A., Jacobs C. W., Cooper C. R., Harris J. L. 1983a; Cell wall changes associated with yeast-to-multicellular form conversion in Wangiella dermatitidis . In Microbiology 1983 pp 239–244 Edited by Schlessinger D. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  19. Szaniszlo P. J., Jacobs C. W., Geis P. A. 1983b; Dimorphism: morphological and biochemical aspects: In Fungi Pathogenic for Humans and Animals (Part A): Biology . pp 323–436 Edited by Howard D. W. New York: Marcel Dekker;
    [Google Scholar]
  20. Taber R. L., Vincent W. S. 1969; Effects of cycloheximide on ribosomal RNA synthesis in yeast. Biochemical and Biophysical Research Communications 34:488–494
    [Google Scholar]
  21. Timberlake W. E., Griffin D. H. 1974; Differential effects of cycloheximide and other inhibitors of protein synthesis on in vivo ribosomal RNA synthesis in Achlya ambisexualis . Biochimica et biophysica acta 353:248–252
    [Google Scholar]
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