1887

Abstract

-Aminobenzoic acid synthase ( synthase) of catalyses the conversion of chorismic acid to -aminobenzoic acid (), a precursor of the aromatic -aminoacetophenone moiety of candicidin, a polyene macrolide antibiotic. This enzyme uses glutamine or ammonia as amino donors for formation. Enzyme extracts converted [C]chorismic acid to labelled synthase was present in 3570 only during the antibiotic producing phase. No detectable levels of the enzyme were found in cell-free extracts of nonproducing mutants of obtained after UV mutagenesis. synthase activity was found also in var. , producer of the polyene macrolide antibiotic fungimycin, but it was not present in extracts of several other streptomycetes that do not produce aromatic polyene macrolide antibiotics. synthase (amidotransferase) activity was partially purified by io-gel and Sephacryl S-200 filtrations. The estimated molecular weight was 50000. synthase was repressed by aromatic amino acids and but not by anthranilic acid. Inorganic phosphate strongly repressed but did not inhibit synthase activity.

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/content/journal/micro/10.1099/00221287-131-6-1279
1985-06-01
2019-10-16
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-6-1279
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