1887

Abstract

Recombinant plasmids were constructed that expressed the KS71A, KS71B and KS71C fimbrial antigens of the pyelonephritogenic strain KS71 (04:K12) in HB101. The KS71C-encoding genes were located on a 6·4 kb dIII–I fragment obtained from the recombinant cosmid pKTH145 that expresses this antigen. Spontaneous KS71C mutants were isolated that contained a 0·8 kb insert in a specific restriction fragment of KS71C-encoding recombinant plasmids. The KS71 B-encoding segment was located on a 11·5 kb deletable DNA fragment of recombinant cosmid pKTH144. A DNA fragment encoding the KS71A fimbria was obtained on a 12 kb RI fragment of the recombinant cosmid expressing this antigen in HB101 and closely resembled the KS71B-encoding fragment. In the recombinant cosmid, the KS71 B-expressing region was flanked by homologous DNA segments. A similar stretch of DNA was found close to the KS71A-expressing DNA region.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-3-571
1985-03-01
2024-10-06
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/3/mic-131-3-571.html?itemId=/content/journal/micro/10.1099/00221287-131-3-571&mimeType=html&fmt=ahah

References

  1. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening of recombinant plasmid DNA. Nucleic Acids Research 7:1513–1517
    [Google Scholar]
  2. Bittner M., Kupfer P., Morris F. C. 1980; Electrophoretic transfer of proteins and nucleic acids from slab gels to diazobenzyloxymethyl cellulose or nitrocellulose sheets. Analytical Biochemistry 102:459–471
    [Google Scholar]
  3. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heynecker H. L., Boyer H. W., Crosa J. H., Falkow S. 1977; Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113
    [Google Scholar]
  4. Brinton C. C. 1965; The structure, function, synthesis, and genetic control of bacterial pili and a model for DNA and RNA transport in Gram-negative bacteria. Transactions of the New York Academy of Sciences 27:1003–1054
    [Google Scholar]
  5. Burrows MR., Sell wood R., Gibbons R. A. 1976; Haemagglutination and adhesive properties associated with the K99 antigen of bovine strains of Escherichia coli. Journal of General Microbiology 96:269–275
    [Google Scholar]
  6. Chang A. C., Cohen S. N. 1978; Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. Journal of Bacteriology 134:1141–1156
    [Google Scholar]
  7. Clegg S., Pierce J. K. 1983; Organization of genes responsible for the production of mannose-resistant nmbriae of a uropathogenic Escherichia coli isolate. Infection and Immunity 42:900–906
    [Google Scholar]
  8. Clewell D. D. 1972; Nature of ColEl plasmid replication in Escherichia coli in the presence of chloramphenicol. Journal of Bacteriology 110:667–678
    [Google Scholar]
  9. Duguid J. P. 1968; The function of bacterial nmbriae. Archivum immunologiae et therapiae experi-mentalis 16:173–188
    [Google Scholar]
  10. Duguid J. P., Smit I. W., Dempster G., Edmunds P. N. 1955; Non-flagellar filamentous appendages (‘nmbriae’) and hemagglutination activity in Bacterium coli. Journal of Pathology and Bacteriology 70:335–348
    [Google Scholar]
  11. Hacker J., Knapp S., Goebel W. 1983; Spontaneous deletions and flanking regions of the chromosomally inherited hemolysin determinant of an Escherichia coli 06 strain. Journal of Bacteriology 154:1145–1152
    [Google Scholar]
  12. Källenius G., Möllby R. 1979; Adhesion of Escherichia coli to human periurethral cells correlated to mannose-resistant hemagglutination of human erythrocytes. FEMS Microbiology Letters 5:296–299
    [Google Scholar]
  13. Källenius G., Möllby R., Svenson S. B., Helin I., Hultberg H., Cedergren B., Winberg J. 1981; Occurrence of P-fimbriated Escherichia coli in urinary tract infections. Lancet ii:1369–1372
    [Google Scholar]
  14. Klemm P., Ørskov I., Ørskov F. 1982; F7 and type-1 like fimbriae from three Escherichia coli strains isolated from urinary tract infection. Injection and Immunity 36:462–468
    [Google Scholar]
  15. Korhonen T. K., Nurmiaho E.-L., Ranta H., Svanborg Eden C. 1980; New method for isolation of immunologically pure pili from Escherichia coli. Infection and Immunity 27:569–575
    [Google Scholar]
  16. Korhonen T. K., Väisänen V., Saxen H., Hult berg H., Svenson S. B. 1982; P-antigen-recognizing fimbriae from human uropathogenic Escherichia coli strains. Infection and Immunity 37:286–291
    [Google Scholar]
  17. Korhonen T. K., Tarkka E., Ranta H., Haahtela K. 1983; Type-3 fimbriae of Klebsiella sp.: Molecular characterization and role in bacterial adhesion to plant roots. Journal of Bacteriology 155:860–865
    [Google Scholar]
  18. Low D., David V., Lark D., Schoolnik G., Falkow S. 1984; Gene clusters governing the production of hemolysin and mannose resistant hemagglutination are closely linked in Escherichia coli serotype 04 and 06 isolates from urinary tract infections. Infection and Immunity 43:353–358
    [Google Scholar]
  19. Mandel M., Higa A. 1970; Calcium dependent bacteriophage DNA infection. Journal of Molecular Biology 53:154–162
    [Google Scholar]
  20. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular cloning: A laboratory manual. Cold Spring Harbor: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Mooi F. R., Harms N., Barker D., De Graaf F. K. 1981; Organization and expression of genes involved in the production of the K88ab antigen. Infection and Immunity 32:1155–1163
    [Google Scholar]
  22. Normark S., Lark D., Hull R., Norgren M., Baga M., O’Hanley P., Schoolnik G., Fal-kow S. 1983; Genetics of a digalactoside binding adhesin from a uropathogenic Escherichia coli strain. Infection and Immunity 41:942–948
    [Google Scholar]
  23. Orskov I., Ørskov F., Birch-Andersen A. 1980; Comparison of Escherichia coli fimbrial antigen F7 with type-1 fimbriae. Infection and Immunity 27:657–666
    [Google Scholar]
  24. Rhen M., Klemm P., Wahlström E., Svenson S. B., Källenius G., Korhonen T. K. 1983a; P-fimbriae of Escherichia coli: Immuno- and protein-chemical characterization of fimbriae from two pyelonephritogenic strains. FEMS Microbiology Utters 18:233–238
    [Google Scholar]
  25. Rhen M., Knowles J., Penttila M. E., Sarvas M., Korhonen T. K. 1983b; P-fimbriae of Escherichia coli: Molecular cloning fragments containing the structural genes. FEMS Microbiology Letters 19:119–123
    [Google Scholar]
  26. Rhen M., Makela P. H., Korhonen T. K. 1983c; P-fimbriae of Escherichia coli are subject to phase variation. FEMS Microbiology Letters 19:267–271
    [Google Scholar]
  27. Rhen M., Wahlström E., Korhonen T. K. 1983d; P-fimbriae of Escherichia coli: Fractionation by immune precipitation. FEMS Microbiology Utters 18:227–232
    [Google Scholar]
  28. Rigby P. J. W., Dieckmann M., Rhodes C., Berg P. 1977; Labelling of deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–241
    [Google Scholar]
  29. Southern E. 1975; Detection of specific sequences among DNA fragments separated by gel electro-phoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  30. Svanborg Eden C., Hanson L. A., Jodal U., Linberg U., Sohl-Akerlund A. 1976; Variable adhesion to normal urinary tract epithelial cells of Escherichia coli strains associated with various forms of urinary tract infection. Lancet ii:490–492
    [Google Scholar]
  31. Väisänen-Rhen V. 1984; Fimbriae-like hemagglutinin of Escherichia coli 075. Infection and Immunity (in the Press)
    [Google Scholar]
  32. Väisänen V., Elo J., Tallgren L. C., Siitonen A., Makela P. H., Svanborg Eden C., Källenius G., Svenson S. B., Hultberg H., Korhonen T. K. 1981; Mannose-resistant hemagglutination and P antigen recognition are characteristics of Escherichia coli causing primary pyelonephritis. Lancet ii:1366–1369
    [Google Scholar]
  33. Van Die I., Van Den Hondel C., Hamstra H.-J., Hoekstra W., Bergmans H. 1983; Studies on the fimbriae of an Escherichia coli 06 : K2 : H1 : F7 strain: Molecular cloning of a DNA fragment encoding a fimbrial antigen responsible for mannose resistant haemagglutination of human erythrocytes. FEMS Microbiology Utters 19:77–82
    [Google Scholar]
  34. Van Die I., Van Megen I., Hoekstra W., Bergmans H. 1984; Molecular organisation of the genes involved in the production of F72 fimbriae, causing mannose resistant haemagglutination, of a uropathogenic Escherichia coli 06:K2:H1:F7 strain. Molecular and General Genetics 194:528–533
    [Google Scholar]
/content/journal/micro/10.1099/00221287-131-3-571
Loading
/content/journal/micro/10.1099/00221287-131-3-571
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error