1887

Abstract

Recombinant plasmids were constructed that expressed the KS71A, KS71B and KS71C fimbrial antigens of the pyelonephritogenic strain KS71 (04:K12) in HB101. The KS71C-encoding genes were located on a 6·4 kb dIII–I fragment obtained from the recombinant cosmid pKTH145 that expresses this antigen. Spontaneous KS71C mutants were isolated that contained a 0·8 kb insert in a specific restriction fragment of KS71C-encoding recombinant plasmids. The KS71 B-encoding segment was located on a 11·5 kb deletable DNA fragment of recombinant cosmid pKTH144. A DNA fragment encoding the KS71A fimbria was obtained on a 12 kb RI fragment of the recombinant cosmid expressing this antigen in HB101 and closely resembled the KS71B-encoding fragment. In the recombinant cosmid, the KS71 B-expressing region was flanked by homologous DNA segments. A similar stretch of DNA was found close to the KS71A-expressing DNA region.

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1985-03-01
2021-08-02
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