%0 Journal Article %A RHEN, MIKAEL %T Characterization of DNA Fragments Encoding Fimbriae of the Uropathogenic Escherichia coli Strain KS71 %D 1985 %J Microbiology, %V 131 %N 3 %P 571-580 %@ 1465-2080 %R https://doi.org/10.1099/00221287-131-3-571 %I Microbiology Society, %X Recombinant plasmids were constructed that expressed the KS71A, KS71B and KS71C fimbrial antigens of the pyelonephritogenic Escherichia coli strain KS71 (04:K12) in E. coli HB101. The KS71C-encoding genes were located on a 6·4 kb HindIII–XhoI fragment obtained from the recombinant cosmid pKTH145 that expresses this antigen. Spontaneous KS71C– mutants were isolated that contained a 0·8 kb insert in a specific restriction fragment of KS71C-encoding recombinant plasmids. The KS71 B-encoding segment was located on a 11·5 kb deletable DNA fragment of recombinant cosmid pKTH144. A DNA fragment encoding the KS71A fimbria was obtained on a 12 kb EcoRI fragment of the recombinant cosmid expressing this antigen in E. coli HB101 and closely resembled the KS71B-encoding fragment. In the recombinant cosmid, the KS71 B-expressing region was flanked by homologous DNA segments. A similar stretch of DNA was found close to the KS71A-expressing DNA region. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-131-3-571