Summary: The influence of the nitrogen source on pyoverdine production by grown in chemostat culture in synthetic media at constant pH was studied. Pigment synthesis was highest in a medium with proline as the nitrogen source; other amino acids were less effective in promoting pigment synthesis. The pH and Fe content of the media affected pyoverdine production. The ratio of pigment to growth was maximal at pH 8. When the Fe concentration was increased from 10 to 200 μg 1 in media with the same nitrogen source, pyoverdine synthesis decreased. Since the Fe contents of the synthetic media were different, the amount present was adjusted to a standard concentration to permit comparisons between the various nitrogen sources.


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