1887

Abstract

Summary: Deletions of the and genes of have been constructed and recombined into the chromosome to produce strains that completely lack penicillin-binding protein 1A or penicillin-binding protein 1B. In each case a DNA fragment internal to the gene was replaced by a fragment encoding an antibiotic resistance. The and deletions can therefore be readily introduced into other strains by P1 transduction of the antibiotic resistance. Although the complete absence of penicillin-binding protein 1A or penicillin-binding protein 1B was tolerated, the absence of both of these proteins was shown to result in bacterial lysis.

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/content/journal/micro/10.1099/00221287-131-10-2839
1985-10-01
2021-05-09
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