1887

Abstract

Summary: A λ 4A derivative carrying the gene of has been identified by transformation of a mutant temperature-sensitive in spore outgrowth. The cloned region is a single RI fragment 14 kb in legnth. In addition to , the cloned DNA includes at least part of the and loci.

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/content/journal/micro/10.1099/00221287-131-10-2831
1985-10-01
2021-05-16
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References

  1. Albertini A. M., Galizzi A. 1975; Mutant of Bacillus subtilis with a temperature-sensitivelesionin ribonucleic acid synthesis during germination. Journal of Bacteriology 124:14–25
    [Google Scholar]
  2. Albertini A. M., Galizzi A. 1982; Pattern of RNA transcription during Bacillus suhtilisspore outgrowth. Journal ofGeneral Microbiology 128:247–253
    [Google Scholar]
  3. Anagnostopoulos C., Spizizen J. 196l; Requirements for transformation in Bacillus subtilis. Journal of Bacteriology 81:741–746
    [Google Scholar]
  4. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  5. Bolivar F. 1978; Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique EcoRI sites for selection of EcoRI generated recombinant DNA molecules. Gene 4:121–136
    [Google Scholar]
  6. Boyer H. W., Roulland-Dussoix D. 1969; Acomplementation analysis of the restriction and modification of DNA in Escherichia coli. Journal of Molecular Biology 41:459–472
    [Google Scholar]
  7. Cohen S. N., Chang A. C. Y., Boyer H. W., Helling R.B. 1973; Construction of biologically functional bacterial plasmids in vitro. Proceedings of the National Academy ofSciences ofthe United States of America 70:3240–3244
    [Google Scholar]
  8. Dagert M., Ehrlich D. 1979; Prolongedincubation in calcium chloride improves the competence of Escherichia coli cells. Gene 6:23–28
    [Google Scholar]
  9. Davis M., Mingioli E. 1950; Mutants of Escherichia coli requiring methionine or vitamin 812. Journal of Bacteriology 60:17–28
    [Google Scholar]
  10. Davis R. W., Simon M., Davidson N. 1971; Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods of Enzymology 21:413–428
    [Google Scholar]
  11. Davis R.W., Botstein D., Rorn J. H. 1980; Rapid A DNA isolation. In Adt>anced Bacterial Genetics: a Manualör Genetic Engineering pp 109–111 New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Ferrari E., Henner D., Hoch J. A. 1981; Isolation of Bacillus subtilis genes from a Charon 4A library. Journal of Bacteriology 146:430–432
    [Google Scholar]
  13. Galizzi A., Gorrini F., Rollier A., Polsinelli M. 1973; Mutants of Bacillus suhtilis temperature sensitive in the outgrowth phase of spore germination. Journal of Bacteriology 113:1482–I490
    [Google Scholar]
  14. Galizzi A., Siccardi A. G., Mazza G., Canosi U., Polsinelli M. 1976; A recombination test to classify mutants of Bacillus subtilis of identical phenotype. Genetical Research 27:47–58
    [Google Scholar]
  15. Leder P., Tiemeier D., Enquist L. 1977; EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lgtWES system. Science 196:175–177
    [Google Scholar]
  16. Moir A. 1983; The isolation of A. transducing phages carrying the citG and gerA genes of Bacillus subtilis. Journal of General Microbiology 129:303–3l0
    [Google Scholar]
  17. Nomura S., Yamane K., Sasaki T., Yamasaki M., Tamura G., Maruo B. 1978; Tunicamycin resistant mutants and chromosomal locations of mutational sites in Bacillus subtilis. Journal of Bacteriology 136:8I8–821
    [Google Scholar]
  18. Parkinson J. S., Huskey R.J. 1971; Deletionmutants of bacteriophage lambda. Isolation and initial characterization. Journal of Molecular Biology 56:369–384
    [Google Scholar]
  19. Pjggot P.J., Moir A., Smith D.A. 1981; Advances in the genetics of Bacillus subtilis differentiation. In Sporulution and Germination pp. 29–39 Edited by Levinson H. S., Sonenshein A.L., Tipper D.J. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Ratcliff S.W., Luh J., Ganesan A.T., Behrens B., Thompson R., Montenegro M. A., Morelli G., Trautner T.A. 1979; The genome of Bacillus subtilis phage SPPI: the arrangement of restriction endonuclease generated fragments. Molecular and General Genetics 168:165–172
    [Google Scholar]
  21. Setlow1 P. 1981; Biochemistry of bacterial forespore development and spore germination. In Sporulution and Germination pp 13–28 Edited by Levinson H.S., Sonenshein A.L., Tipper D.J. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  22. Spizjzen J. l958; Transformation of biochemically deficientstrains of Bacillus subtilis by deoxyribo nucleate. Proceedings of the National Academy of Sciences of the United Stutes of America 44:1072–1078
    [Google Scholar]
  23. Yang M., Galizzi A., Henner D. I983; Nucleotide sequence of the amylase gene from Bacillus subtilis. Nudeic Acids Research 11:237–249
    [Google Scholar]
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