1887

Abstract

Acid-soluble and alkali-insoluble glucan fractions were prepared from yeast, hyphal and germ-tube forming cells of Alkali-insoluble glucan was also extracted from purified yeast cell walls. Paper chromatography of partial acid hydrolysates confirmed that the glucan preparations contained β(1→3)-and (1→6)-chains but no mixed intra-chain (1→3)/(1→6) linkages. Methylation and C-NMR analyses showed that the acid-soluble glucan consisted of a highly branched polymer composed mainly (67·0% to 76·6%) of (1→6)-linked glucose residues. The alkali-insoluble glucan from yeast and hyphal cells contained from 29·6% to 38·9% (1→3) and 43·3% to 53·2% (1→6) linkages. Alkali-insoluble glucan from germ-tube forming cells consisted of 67·0% (1→3) and 14% (1→6) linkages. Branch points accounted for 6·7%, 12·3% and 17·4% of the residues in the alkali-insoluble glucan of yeast, germ-tube forming and hyphal cells, respectively.

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1984-12-01
2021-08-04
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