1887

Abstract

Acid-soluble and alkali-insoluble glucan fractions were prepared from yeast, hyphal and germ-tube forming cells of Alkali-insoluble glucan was also extracted from purified yeast cell walls. Paper chromatography of partial acid hydrolysates confirmed that the glucan preparations contained β(1→3)-and (1→6)-chains but no mixed intra-chain (1→3)/(1→6) linkages. Methylation and C-NMR analyses showed that the acid-soluble glucan consisted of a highly branched polymer composed mainly (67·0% to 76·6%) of (1→6)-linked glucose residues. The alkali-insoluble glucan from yeast and hyphal cells contained from 29·6% to 38·9% (1→3) and 43·3% to 53·2% (1→6) linkages. Alkali-insoluble glucan from germ-tube forming cells consisted of 67·0% (1→3) and 14% (1→6) linkages. Branch points accounted for 6·7%, 12·3% and 17·4% of the residues in the alkali-insoluble glucan of yeast, germ-tube forming and hyphal cells, respectively.

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1984-12-01
2024-04-26
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References

  1. Bacon J.S.D. 1979; Nature and disposition of polysaccharides within the cell envelope. In Yeast Cell Envelopes: Biochemistry, Biophysics and Ultrastructure I: pp. 66–84
    [Google Scholar]
  2. Ballou C.E. 1982; Yeast cell wall and cell surface. In Molecular Biology of the Yeast Saccharomyces: Metabolism and Gene Expression pp. 335–360 Strathern J. N., Jones E. W., Broach J. R. Edited by Cold Spring Harbor: Cold Spring Harbor Laboratory;
    [Google Scholar]
  3. Bassieux D., Gagnaire D., Vignon M. 1967; Etude par R.M.N.-13C et lH du (1𡤢6)-β-D-glucane et des oligosaccharides lineaires et cycliquescorrespon-dants. Carbohydrate Research 56:19–33
    [Google Scholar]
  4. Bishop C.T., Blank F., Gardner P.E. 1960; The cell wall polysaccharides of Candida albicans:glucan, mannan and chitin. Canadian Journal of Chemistry 38:869–881
    [Google Scholar]
  5. Braun P.C., Calderone R.A. 1978; Chitin synthesis in Candida albicans: comparison of yeast and hyphal forms. Journal of Bacteriology 133:1472–1477
    [Google Scholar]
  6. Cabib E., Roberts R., Bowers B. 1982; Synthesis of the yeast cell wall and its regulation. Annual Review of Biochemistry 51:763–793
    [Google Scholar]
  7. Cassone A., Simonetti N., Strippoli V. 1973; Ultrastructural changes in the wall during germ-tube formation from blastospores in Candida albicans. Journal of General Microbiology 77:417–426
    [Google Scholar]
  8. Chattaway F.W., Holmes M.R., Barlow A.J.E. 1968; Cell wall composition of the mycelial and blastospore forms of Candida albicans. Journal of General Microbiology 51:367–376
    [Google Scholar]
  9. Colson P., Jennings H.J., Smith I.C. 1974; Composition, sequence and conformation of polymers and oligomers of glucose as revealed by Carbon-13 Nuclear Magnetic Resonance. Journal of the American Chemical Society 96: 26:8081–8087
    [Google Scholar]
  10. Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  11. Duffus J.H., Levi C., Manners D.J. 1982; Yeast cell wall glucans. Advances in Microbial Physiology 23:151–181
    [Google Scholar]
  12. Gale E.F., Ingram J., Kerridge D., Notario V., Wayman F. 1980; Reduction of amphotericin resistance in stationary phase cultures of Candida albicans by treatment with enzymes. Journal of General Microbiology 117:383–391
    [Google Scholar]
  13. Gopal P.K., Sullivan P.A., Shepherd M.G. 1984; Isolation and structure of glucan from regenerating spheroplasts of Candida albicans. Journal of General Microbiology 130:1217–1225
    [Google Scholar]
  14. Kreger D.R., Kopecká M. 1975; On the nature and formation of the fibrillar nets produced by protoplasts of Saccharomyces cerevisiae in liquid media: an electronmicroscopic, X-ray diffraction and chemical study. Journal of General Microbiology 92:207–220
    [Google Scholar]
  15. Manners D.J., Masson A.J., Patterson J.C. 1973a; THE structure of β(1→3)-d-glucan from yeast cell walls. Biochemical journal 135:19–30
    [Google Scholar]
  16. Manners D.J., Masson A.J., Patterson J.C., Bjorndal H., Lindberg B. 1973b; The structure of a β(1→6)-d-glucan from yeast cell walls. Biochemical Journal 135:31–36
    [Google Scholar]
  17. Poulter R., Jeffery K., Hubbard M.J., Sullivan P.A., Shepherd M.G. 1981; Parasexual genetic analysis of Candida albicans by spheroplast fusion. Journal of Bacteriology 146:833–840
    [Google Scholar]
  18. Ram S., Beyer R., Shepherd M.G., Sullivan P.A. 1981; Isolation and analysis of neutral glucans from Eckloniaradiata and Cystophorascalaris. Carbohydrate Research 96:95–104
    [Google Scholar]
  19. Rees D.A. 1973; Polysaccharide conformation. In Carbohydrates, MTP International Review of Science: Organic Chemistry series I 7 pp. 251–283 Aspinall G. O. Edited by London: Butterworths;
    [Google Scholar]
  20. Saito H., Ohki T., Takasuka N., Sasaki T. 1977; A 13C-NMR spectral study of a gel forming branched (1→3)-β-d-glucan, (1entinan) from Lentinusedodes and its acid-degraded fractions. Structure and dependence of conformation on molecular weight. Carbohydrate Research 58:293–305
    [Google Scholar]
  21. Scherwitz C., Martin R., Ueberberg H. 1978; Ultrastructural investigations of the formation of Candida albicans germ-tubes and septa. Sabouraudia 16:115–124
    [Google Scholar]
  22. Shepherd M.G., Sullivan P.A. 1976; The production and growth characteristics of yeast and mycelial forms of Candida albicans in continuous culture. Journal of General Microbiology 93:361–370
    [Google Scholar]
  23. Shepherd M.G., Chiew Y.Y., Ram S.P., Sullivan P.A. 1980; Germ-tube induction in Candida albicans. Canadian Journal of Microbiology 26:21–26
    [Google Scholar]
  24. Sietsma J.H., Wessels J.G.H. 1981; Solubility of (1→3)-β-d/(1→6)-β-D-glucan in fungal walls: importance of presumed linkage between glucan and chitin. Journal of General Microbiology 125:209–212
    [Google Scholar]
  25. Stewart P.R. 1975; Analytical methods for yeasts. Methods in Cell Biology 12:111–147
    [Google Scholar]
  26. Sullivan P.A., Chiew Y.Y., Molloy C., Templeton M.D., Shepherd M.G. 1983; An analysis of the metabolism and cell wall composition of Candida albicans during germ-tube formation. Canadian Journal of Biochemistry 29:1514–1525
    [Google Scholar]
  27. Sullivan P.A., Mchugh N.J., Romana L.K., Shepherd M.G. 1984; The secretion of N-acetylglucosaminidase during germ-tube formation in Candida albicans. Journal of General Microbiology 130:2213–2218
    [Google Scholar]
  28. Tronchin G., Poulain D., Herbaut J., Biguet J. 1981; Localization of chitin in the cell wall of Candida albicans by means of wheat germ agglutinin. Fluorescence and ultrastructural studies. European Journal of Cell Biology 26:121–128
    [Google Scholar]
  29. Yu R.J., Bishop C.T., Cooper F.P., Hasenclever H.F., Blank F. 1967; Structural studies of mannans from Candida albicans(serotypes A, B), Candida parapsilosis, Candida stellatoidea&Candida tropicalis. . Canadian Journal of Chemistry 45:2207–2211
    [Google Scholar]
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