@article{mbs:/content/journal/micro/10.1099/00221287-130-12-3295, author = "Gopal, Pramod K. and Shepherd, Maxwell G. and Sullivan, Patrick A.", title = "Analysis of Wall Glucans from Yeast, Hyphal and Germ-tube Forming Cells of Candida albicans", journal= "Microbiology", year = "1984", volume = "130", number = "12", pages = "3295-3301", doi = "https://doi.org/10.1099/00221287-130-12-3295", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-130-12-3295", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Acid-soluble and alkali-insoluble glucan fractions were prepared from yeast, hyphal and germ-tube forming cells of Candida albicans. Alkali-insoluble glucan was also extracted from purified yeast cell walls. Paper chromatography of partial acid hydrolysates confirmed that the glucan preparations contained β(1→3)-and β(1→6)-chains but no mixed intra-chain β(1→3)/β(1→6) linkages. Methylation and 13C-NMR analyses showed that the acid-soluble glucan consisted of a highly branched polymer composed mainly (67·0% to 76·6%) of β(1→6)-linked glucose residues. The alkali-insoluble glucan from yeast and hyphal cells contained from 29·6% to 38·9% β(1→3) and 43·3% to 53·2% (1→6) linkages. Alkali-insoluble glucan from germ-tube forming cells consisted of 67·0% β(1→3) and 14% β(1→6) linkages. Branch points accounted for 6·7%, 12·3% and 17·4% of the residues in the alkali-insoluble glucan of yeast, germ-tube forming and hyphal cells, respectively.", }