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Mutualistically associated bacteria were isolated from two genera of nematodes: Xenorhabdus nematophilus from four species of Steinernema (= Neoaplectana) and X. luminescens from two species of Heterorhabditis. Mutualistic bacteria were also isolated from two unidentified species of heterorhabditid nematodes and were partially characterized by selected standard bacteriological tests. The soluble proteins obtained from the primary and secondary forms of these bacteria were subjected to electrophoretic examination on acrylamide gels. Total protein profiles and certain isoenzyme electrophoregrams were compared. The results revealed distinct subspecific differences in profiles of X. nematophilus and suggested new subspecific groupings in X. luminescens.
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