1887

Abstract

The synthesis, activity and breakdown of the denitrifying enzymes of and were found to be regulated by O. Nitrogen oxide reductases were present in anaerobically grown and symbiotic , but in the case of organisms that had been grown aerobically the enzymes were induced only after a period of incubation under anaerobic conditions. Activity of the denitrification system that had been induced in aerobically grown cells was inhibited by O. Denitrification by anaerobically grown cells and bacteroids was stimulated by 5% O. Air inhibited denitrification completely. Little loss of denitrifying activity was shown by cells incubated in 5% O, but cells incubated at ⩾10% O showed a rapid loss of denitrification activity.

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1983-08-01
2021-10-19
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References

  1. Antoun H., Bordeleau L.M., Prevost D., Lachance R.A. 1980; Absence of a correlation between nitrate reductase and symbiotic nitrogen fixation efficiency in Rhizobium meliloti.. Canadian Journal of Plant Science 60:209–212
    [Google Scholar]
  2. Appleby C.A. 1969; Electron transport systems of Rhizobium japonicum I. Haemoprotein P-450, other CO-reactive pigments, cytochromes and oxidases in bacteroids from N2-fixing root nodules. Biochimica et biophysica acta 172:71–87
    [Google Scholar]
  3. Appleby C.A. 1974; Leghemoglobin. In The Biology of Nitrogen Fixation pp. 521–554 Quispel A. Edited by Amsterdam: North Holland Publishing Co.;
    [Google Scholar]
  4. Bergersen F.J. 1961; Nitrate reductase in soybean root nodules. Biochimica et biophysica acta 52:206–207
    [Google Scholar]
  5. Betlach M.R., Tiedje J.M. 1981; Kinetic explanation for accumulation of nitrite, nitric oxide and nitrous oxide during bacterial denitrification. Applied and Environmental Microbiology 42:1074–1084
    [Google Scholar]
  6. Burris R.J. 1979; Inhibition. In A Treatise on Dinitrogen Fixation, Section II Biochemistry pp. 569–604 Hardy R.W.F., Burns R.C. Edited by New York: John Wiley.;
    [Google Scholar]
  7. Cheniae G., Evans H.J. 1960; Physiological studies on nodule-nitrate reductase. Plant Physiology 35:454–462
    [Google Scholar]
  8. Daniel R.M., Appleby C.A. 1972; Anaerobicnitrate, symbiotic and aerobic growth of Rhizobium japonicum: effects on cytochrome P-450, other haemoproteins, nitrate and nitrite reductases. Biochimica et biophysica acta 275:347–354
    [Google Scholar]
  9. Daniel R.M., Gray J. 1976; Nitrate reductase from anaerobically grown Rhizobium japonicum.. Journal of General Microbiology 96:247–251
    [Google Scholar]
  10. Daniel R.M., Steele K.W., Limmer A.W. 1980a; Denitrification by rhizobia. A possible factor contributing to nitrogen losses from soils. New Zealand Agricultural Science 14:109–112
    [Google Scholar]
  11. Daniel R.M., Smith I.M., Phillip J.A.D., Ratcliffe H.D., Drozd J.W., Bull A.T. 1980b; Anaerobic growth and denitrification by Rhizobium japonicum and other rhizobia. Journal of General Microbiology 120:157–521
    [Google Scholar]
  12. Daniel R.M., Limmer A.W., Steele K.W., Smith I.M. 1982; Anaerobic growth, nitrate reduction and denitrification in 46 rhizobium strains. Journal of General Microbiology 128:1811–1815
    [Google Scholar]
  13. Dart P. 1977; Infection and development of leguminous nodules. In A Treatise on Dinitrogen Fixation, Section 3, Biology pp. 367–472 Hardy R.W.F., Silver W.S. Edited by New York: John Wiley.;
    [Google Scholar]
  14. Dazzo F.B., Brill W.J. 1978; Regulation by fixed nitrogen of host-symbiont recognition in the Rhizobium - clover symbiosis. Plant Physiology 62:18–21
    [Google Scholar]
  15. Drew M.C., Lynch J.M. 1980; Soil anaerobiosis, microorganisms and root function. Annual Review of Phytopathology 18:37–66
    [Google Scholar]
  16. Dunstan R.J., Kelley B.C., Nicholas D.J.D. 1982; The recycling of N2 and H2 in a denitrifying phototrophic bacterium. 12th International Congress of Biochemistry, Perth, Western Australia p. 154: abstract
    [Google Scholar]
  17. Gibson A.H. 1977; The influence of the environment and managerial practices on the legume-Rhizobium symbiosis. In A Treatise on Dinitrogen Fixation, Section 4, Agronomy and Ecology pp. 393–450 Hardy R.W.F., Gibson A.H. Edited by New York: John Wiley.;
    [Google Scholar]
  18. Gibson A.H., Pagan J.D. 1977; Nitrate effects on the nodulation of legumes inoculated with nitrate-reductase-deficient mutants of Rhizobium. Planta 134:17–22
    [Google Scholar]
  19. Goa J. 1953; A micro biuret method for protein determination. Determination of total protein in cerebrospinal fluid. Scandinavian Journal of Clinical and Laboratory Investigations 5:218–222
    [Google Scholar]
  20. Hewitt E.J. 1966 Sand and Water Culture Methods Used in the Study of Plant Nutrition. Farnham Royal, (Bucks): Commonwealth Agricultural Bureaux;
    [Google Scholar]
  21. Justin P., Kelly D.P. 1978; Metabolic changes in Thiobacillus denitrificans accompanying the transition from aerobic to anaerobic growth in continuous culture. Journal of General Microbiology 107:131–137
    [Google Scholar]
  22. Kaspar H.F., Tiedje J.M. 1980; Response of electron-capture detector to hydrogen, oxygen, nitrogen, carbon dioxide, nitric oxide and nitrous oxide. Journal of Chromatography 193:142–147
    [Google Scholar]
  23. Kennedy I.R., Rigaud J., Trinchant J.C. 1975; Nitrate reductase from bacteroids of Rhizobium japonicum: Enzyme characteristics and possible interaction with nitrogen fixation. Biochimica et biophysica acta 397:24–35
    [Google Scholar]
  24. Knowles R. 1982; Denitrification. Microbiological Reviews 46:43–70
    [Google Scholar]
  25. Manhart J.R., Wong P.P. 1980; Nitrate effect on nitrogen fixation (acetylene reduction) activities of legume root nodules induced by rhizobia with varied nitrate reductase activities. Plant Physiology 65:502–505
    [Google Scholar]
  26. Mozen M.M., Burris R.H. 1954; The incorporation of 15N-labelled nitrous oxide by nitrogen fixing agents. Biochimica et biophysica acta 14:577–578
    [Google Scholar]
  27. Murphy S.G., Elkan G.H. 1965; Nitrogen metabolism of some strains of Rhizobium japonicumhaving different nodulating capacities. Canadian Journal of Microbiology 11:1039–1041
    [Google Scholar]
  28. Ohyama T., Kumazawa K. 1979; Assimilation and transport of nitrogenous compounds originated from l5N2 fixation and 15NO3 absorption. Soil Science and Plant Nutrition 25:9–19
    [Google Scholar]
  29. Pate J.S. 1977; Nodulation and nitrogen metabolism. In The Physiology of the Garden Pea pp. 349–383 Sutcliffe J.F., Pate J.S. Edited by London: Academic Press.;
    [Google Scholar]
  30. Paul E.A., Victoria R.L. 1978; Nitrogen transfers between the soil and atmosphere. In Environmental Biogeochemistry and Geomicrobiology, volume 2, The Terrestrial Environment pp. 525–542 Krumbein W.E. Edited by Michigan: Ann Arbor Science;
    [Google Scholar]
  31. Payne W.J. 1981 Denitrification. New York: John Wiley.;
    [Google Scholar]
  32. Picci G., Lepidi A.A. 1967; Alcuni aspetti della riduzione dei nitrati a nitriti ad opera di Rhizobium leguminosarum Frank. L’Agricoltura Ilaliana 66:21–27
    [Google Scholar]
  33. Randall D.D., Russell W.J., Johnson D.R. 1978; Nodule nitrate reductase as a source of reduced nitrogen in soybean, Glycine max. Physiolo-gia plantarum 44:325–328
    [Google Scholar]
  34. Rigaud J., Bergersen F.J., Turner G.L., Daniel R.M. 1973; Nitrate dependent anaerobic acetylene-reduction and nitrogen-fixation by soybean bacteroids. Journal of General Microbiology 77:137–144
    [Google Scholar]
  35. Sacks L.E., Barker H.A. 1949; The influence of oxygen on nitrate and nitrite reduction. Journal of Bacteriology 58:11–22
    [Google Scholar]
  36. Sik T., Kondorosi A., Barabas I., Svab Z. 1976; Nitrate reductase and effectiveness in Rhizobium. In Symposium on Nitrogen Fixation 2 pp. 374–382 Newton W.E., Nyman C.J. Edited by Washington State University Press:
    [Google Scholar]
  37. Smith G.S., Edmonds A.S., Middleton K.R. 1980; Effects of chloramphenicol on growth, chemical composition and chlorophyll content of intact plants. New Phytologist 86:181–189
    [Google Scholar]
  38. Smith K.A. 1980; A model of the extent of anaerobic zones in aggregated soils, and its potential application to estimates of denitrification. Journal of Soil Science 31:263–277
    [Google Scholar]
  39. Streeter J.G. 1982; Synthesis and accumulation of nitrite in soybean nodules supplied with nitrate. Plant Physiology 69:1429–1434
    [Google Scholar]
  40. Trinchant J.C., Rigaud J. 1981; Acetylene reduction and respiration of bacteroids isolated from French-beans receiving nitrate. Physiologia plantarum 53:511–517
    [Google Scholar]
  41. Trinchant J.C., Rigaud J. 1982; Nitrite and nitric oxide as inhibitors of nitrogenase from soybean bacteroids. Applied and Environmental Microbiology 44:1385–1388
    [Google Scholar]
  42. Vance C.P., Heichel G.H. 1981; Nitrate assimilation during vegetative regrowth of alfalfa. Plant Physiology 68:1052–1056
    [Google Scholar]
  43. Vasconcelos L.De., Miller L., Neyra C.A. 1980; Free-living and symbiotic characteristics of chlorate-resistant mutants of Rhizobium japonicum. Canadian Journal of Microbiology 26:338–342
    [Google Scholar]
  44. Zablotowicz R.M., Focht D. D. 1979; Denitrification, and anaerobic, nitrate-dependent acetylene reduction in cowpea rhizobium. Journal of General Microbiology 111:445–448
    [Google Scholar]
  45. Zablotowicz R.M., Eskew D.L., Focht D.D. 1978; Denitrification in Rhizobium. Canadian Journal of Microbiology 24:757–760
    [Google Scholar]
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