1887

Abstract

Lignin degradation by was investigated by culturing the fungus on spruce sapwood chips and extracting the extracellular proteins produced by an active lignin-degrading culture. The conditions for optimum ligninolytic activity were determined using a cultivation technique which simulated natural conditions, involving the use of cellulose in a growth medium placed beneath a lignin-impregnated glass fibre disc that served as a solid-phase support for hyphal growth. The extracellular proteins from the wood chip culture were shown to cause some modification of isolated milled wood lignin isolated from milled wood, namely an increase in hydroxyl groups in the molecule and a slight reduction in the mean molecular weight of the lignin polymers.

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/content/journal/micro/10.1099/00221287-129-7-2103
1983-07-01
2021-05-09
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References

  1. Ander P., Eriksson K.E., MÅnsson P., Pettersson B. 1981; Lignin degradation by Sporotrichum pulverulentum: a new cultivation method to study fungal lignin degradation. International Symposium on Wood and Pulping Chemistry, Stockholm, Sweden 3:71–74
    [Google Scholar]
  2. Ander P., Hatakka A., Eriksson K.E. 1980; Vanillic acid metabolism by the white-rot fungus Sporotrichum pulverulentum. Archives of Microbiology 125:189–202
    [Google Scholar]
  3. Björkman A. 1956; Studies on finely divided wood. Part 1. Extraction of lignin with neutral solvents. Svensk Papperstidning 59:477–485
    [Google Scholar]
  4. FÅhraeus G., Reinhammer B. 1967; Large scale production and purification of laccase from cultures of the fungus Polyporus versicolor and some properties of laccase A. Acta chemica scandinavica 21:2367–2378
    [Google Scholar]
  5. Farmer V.C., Henderson M.E.K., Russell J.D. 1960; Aromatic-alcohol oxidase activity in the growth medium of Polystictus versicolor. Biochemical Journal 74:257–262
    [Google Scholar]
  6. Ishihara T. 1980; The role of laccase in lignin biodegradation. In Lignin Biodegradation: Microbiology, Chemistry and Potential Applications 2 pp. 17–31 Kirk T.K., Higuchi T., Chang H-M. Edited by Florida:: CRC Press.;
    [Google Scholar]
  7. Janshekar H., Fiechter A. 1982; On the bacterial degradation of lignin. European Journal of Applied Microbiology and Biotechnology 14:47–50
    [Google Scholar]
  8. Keyser P., Kirk T.K., Zeikus J.G. 1978; Ligninolytic enzyme system of Phanerochaete chrysosporium: synthesised in the absence of lignin in response to nitrogen starvation. Journal of Bacteriology 135:790–797
    [Google Scholar]
  9. Kirk T.K., Chang H.M. 1975; Decomposition of lignin by white-rot fungi. II. Characterization of heavily degraded lignins from decayed spruce. Holzforschung 29:56–64
    [Google Scholar]
  10. Kirk T.K., Connors W.J., Zeikus J.G. 1976; Requirement for a growth substrate during lignin decomposition by two wood-rotting fungi. Applied and Environmental Microbiology 32:192–194
    [Google Scholar]
  11. Kirk T.K., Schultz E., Connors W.J., Lorenz L.F., Zeikus J.G. 1978; Influence of culture parameters on lignin metabolism by Phanerochaete chrysosporium. Archives of Microbiology 117:277–285
    [Google Scholar]
  12. Lenz B.L. 1968; Application of nuclear magnetic resonance spectroscopy to characterization of lignin. Tappi 51:511–519
    [Google Scholar]
  13. Rosenberg S.L. 1980; Patterns of diffusibility of lignin and carbohydrate-degrading systems in wood-rotting fungi. Mycologia 72:798–812
    [Google Scholar]
  14. Westermark U., Eriksson K.E. 1974; Carbohy-drate-dependent enzymic quinone reduction during lignin degradation. Acta chemica scandinavica 28:204–208
    [Google Scholar]
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