SUMMARY: Lignin degradation by was investigated by culturing the fungus on spruce sapwood chips and extracting the extracellular proteins produced by an active lignin-degrading culture. The conditions for optimum ligninolytic activity were determined using a cultivation technique which simulated natural conditions, involving the use of cellulose in a growth medium placed beneath a lignin-impregnated glass fibre disc that served as a solid-phase support for hyphal growth. The extracellular proteins from the wood chip culture were shown to cause some modification of isolated milled wood lignin isolated from milled wood, namely an increase in hydroxyl groups in the molecule and a slight reduction in the mean molecular weight of the lignin polymers.


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