1887

Abstract

Spheroplasts were prepared from two carotenoid pigment mutants of named NgR and A which were obtained by the chemical mutagenesis of the wild type strain A with -methyl-′-nitro--nitrosoguanidine. The carotenoid pigments and the a- and -mycolic acids were taken as genetic markers and the recombinants were selected on the basis of their colour on Lwenstein-Jensen medium. Spheroplasts of the two mutants were mixed in a 1:1 ratio and were treated with 40 % (w/v) polyethylene glycol 6000 for 5 min at 37 C. The frequency of NgR A recombination in optimal conditions was about 2·5 × 10. The recombinants selected on the basis of their carotenoid pigment profile were also tested for their - and -mycolic acids as a second genetic marker. The results were further confirmed by electron microscopy. The optimal conditions, for spheroplast fusion as a mode of genetic recombination in are described.

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1983-04-01
2022-01-16
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