1887

Abstract

Hybrids were produced by protoplast fusion between strains of and mitotic master strains of with a genetic marker on each linkage group. Analysis of segregants induced by growth on benomyl revealed recombination between every pair of unlinked markers. Parental combinations of markers were often recovered at significantly higher frequencies than expected. This aberrant segregation was not correlated with any particular pair of linkage-groups and was attributed to inter-species incompatibility. The segregation of genetic markers of from the hybrids suggested that and may differ in haploid chromosome number and chromosome size. In sexual crosses between and strains containing chromosomes of mixed parental origin recombinants were recovered. The results support the classification of and as separate species.

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1983-11-01
2021-10-28
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References

  1. AnnÉ J., Peberdy J.F. 1976; Induced fusion of fungal protoplasts following treatment with polyethylene glycol. Journal of General Microbiology 92:413–417
    [Google Scholar]
  2. Bainbridge B.W. 1974; A simple and rapid technique for obtaining a high proportion of hybrid cleistothecia in Aspergillus nidulans. Genetical Research 23:115–117
    [Google Scholar]
  3. Barratt R.W., Ogata W.N., Käfer E. 1975; Aspergillus stock list (Fungal Genetics Stock Centre). Aspergillus Newsletter 13:23–58
    [Google Scholar]
  4. Becarri E., Modigliani P., Morpugo G. 1967; Induction of inter- and intragenic mitotic recombination by 5-fluorodeoxyuridine and 5-fluorouracil in Aspergillus nidulans. Genetics 56:7–12
    [Google Scholar]
  5. Bradshaw R.E., El-Mesillati M., Peberdy J.F. 1983; Genetic analysis in Aspergillus rugulosususing the parasexual cycle. Transactions of the British Mycological Society 81:15–19
    [Google Scholar]
  6. Butcher A.C. 1968; The relationship between sexual outcrossing and heterokaryon incompatibility in Aspergillus nidulans. Heredity 23:443–452
    [Google Scholar]
  7. Croft J.H., Jinks J.L. 1977; Aspects of the population genetics of Aspergillusnidulans. In Genetics and Physiology of Aspergillus pp. 339–360 Smith J.E., Pateman J.A. Edited by New York:: Academic Press.;
    [Google Scholar]
  8. Dales R.B.G., Croft J.H. 1977; Protoplast fusion and the isolation of heterokaryons and diploids from vegetative incompatible strains of Aspergillus nidulans. FEMS Microbiology Letters 1:201–204
    [Google Scholar]
  9. Dales R.B.G., Croft J.H. 1979; Protoplast fusion and the genetic analysis of vegetative incompatibility in Aspergillus nidulans. In Advances in Protoplast Research pp. 73–84 Ferenczy L., Farkas G.L. Edited by Oxford:: Pergamon Press.;
    [Google Scholar]
  10. El-Mesillati M. 1981 Genetic studies on Aspergillus rugulosus. Ph.D. thesis. University of Nottingham.:
    [Google Scholar]
  11. Ferenczy L. 1976; Some characteristics of intra- and interspecific protoplast fusion products of Aspergillus nidulans and Aspergillus fumigatus. In Cell Genetics in Higher Plants pp. 171–181 Dudits D., Farkas G.L., Maliga P. Edited by Budapest:: Akademiai Kiado.;
    [Google Scholar]
  12. Ferenczy L. 1981; Microbial protoplast fusion. Symposia of the Society for General Microbiology 31:1–34
    [Google Scholar]
  13. Ferenczy L., Szegedi M., Kevei F. 1977; Interspecific protoplast fusion and complementation in Aspergilli. Experientia 33:184–186
    [Google Scholar]
  14. Forbes E. 1959; Use of mitotic segregation for assigning genes to linkage groups in Aspergillus nidulans. Heredity 13:67–80
    [Google Scholar]
  15. Hamlyn P.F., Bradshaw R.E., Mellon F.M., Santiago C.M., Wilson J.M., Peberdy J.F. 1981; Efficient protoplast isolation from fungi using commercial enzymes. Enzyme and Microbial Technology 3:321–325
    [Google Scholar]
  16. Kôfer E. 1961; The processes of spontaneous recombination in vegetative nuclei of Apergillus nidulans. Genetics 46:1581–1609
    [Google Scholar]
  17. Kôfer E. 1965; The origins of translocations in Aspergillus nidulans. Genetics 52:217–232
    [Google Scholar]
  18. Kôfer E. 1977; Meiotic and mitotic recombination in Aspergillus and its chromosomal aberrations. Advances in Genetics 19:103–131
    [Google Scholar]
  19. Kevei F., Peberdy J.F. 1977; Interspecific hybridization between Aspergillus nidulans and Aspergillus rugulosus by fusion of somatic protoplasts. Journal of General Microbiology 102:255–262
    [Google Scholar]
  20. Kevei F., Peberdy J.F. 1979; Induced segregation in interspecific hybrids of Aspergillusnidulansand Aspergillus rugulosus obtained by protoplast fusion. Molecular and General Genetics 170:213–218
    [Google Scholar]
  21. Morrison K.B., Ball C. 1978; Gene order on linkage group I in Penicillium chrysogenum. Microbial Genetics Bulletin 45:1–3
    [Google Scholar]
  22. Pontecorvo G. 1956; The parasexual cycle in fungi. Annual Review of Microbiology 10:393–400
    [Google Scholar]
  23. Pontecorvo G., Kôfer E. 1958; Genetic analysis based on mitotic recombination. Advances in Genetics 9:71–104
    [Google Scholar]
  24. Pontecorvo G., Roper J.A., Hemmons D.W., Macdonald K.D., Bufton A.W. 1953; The genetics of Aspergillus nidulans. Advances in Genetics 5:141–238
    [Google Scholar]
  25. Upshall A., Giddings B., Mortimore D. 1977; The use of benlate for distinguishing between haploid and diploid strains of Aspergillus nidulansand Aspergillus terreus. Journal of General Microbiology 100:413–418
    [Google Scholar]
  26. Wood S., Kôfer E. 1969; Effects of UV-irradiation on heterozygous diploids of Aspergillus nidulans I:UV-induced mitotic crossing-over. Genetics 62:507–518
    [Google Scholar]
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