1887

Abstract

Plasmid pMT-trp was constructed by digestion of RSF2124-trp with restriction endonuclease I and ligation with T4 ligase. In pMT-trp about 78% of the DNA of transposon TnA from RSF2124-trp was deleted, and hence the gene for ampicillin resistance was lost. All Trp segregants from pMT-trp carriers in W3110 and its derivatives were found to have lost the entire plasmid. On the other hand, deletion plasmids which had lost the operon were found among Trp segregants from RSF2124-trp carriers, particularly from the mutant strain The experimental fact that deletion occurred exclusively in RSF2124-trp suggests that the presence of TnA in the plasmid (RSF2124-trp) was responsible for the deletion.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-127-1-93
1981-11-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/127/1/mic-127-1-93.html?itemId=/content/journal/micro/10.1099/00221287-127-1-93&mimeType=html&fmt=ahah

References

  1. Bukhari A. I., Shapiro J. A., Adhya S. L. (editors) 1977 DNA Insertion Elements, Plasmids, and Episomes pp. 680–713 New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  2. Clewell D. B. 1972; Nature of ColE1 plasmid replication in Escherichia coli in the presence of chloramphenicol. Journal of Bacteriology 110:667–676
    [Google Scholar]
  3. Collins J., Brüning H. J. 1978; Plasmids useable as gene-cloning vectors in an in vitro packaging by coliphage λ: ‘Cosmids’. Gene 4:85–107
    [Google Scholar]
  4. Dougan G., Saul M., Warren G., Sherratt D. 1978; A functional map of plasmid ColE1. Molecular and General Genetics 158:325–327
    [Google Scholar]
  5. Gelfand D. H., Shepard H. M., D’Farrell P. H., Polisky B. 1978; Isolation and characterization of a ColE1-derived plasmid copy-number mutant. Proceedings of the National Academy of Sciences of the United States of America 75:5869–5873
    [Google Scholar]
  6. Heffron F., Bedinger P., Champoux J. J., Falkow S. 1977; Deletion affecting the transposition of an antibiotic resistance gene. Proceedings of the National Academy of Sciences of the United States of America 74:702–706
    [Google Scholar]
  7. Hershfield V., Boyer H. W., Yanofsky C., Lovett M. A., Helinski D. R. 1974; Plasmid ColE1 as a molecular vehicle for cloning and amplification of DNA. Proceedings of the National Academy of Sciences of the United States of America 71:3455–3459
    [Google Scholar]
  8. Holmans P., Anderson G. C., Clowes R. C. 1978; TnA-directed deletions and translocations within the R6K plasmid. In Microbiology 1978 pp. 38–41 Schlessinger D. Edited by Washington, D.C.: American Society for Microbiology.;
    [Google Scholar]
  9. Imanaka T., Tsunekawa H., Aiba S. 1980; Phenotypic stability of trp operon recombinant plasmids in Escherichia coli. . Journal of General Microbiology 118:253–261
    [Google Scholar]
  10. Kaiser A. D. 1957; Mutations in a temperate bacteriophage affecting its ability to lysogenize Escherichia coli. . Virology 3:42–61
    [Google Scholar]
  11. Kleckner N. 1977; Translocatable elements in procaryotes. Cell 11:11–23
    [Google Scholar]
  12. Matsubara K. 1972; Plasmid formation from bacteriophage λ as a result of interference by resident plasmid λdv. . Virology 47:618–627
    [Google Scholar]
  13. Nagahari K. 1978; Deletion plasmids from the transformation of Pseudomonas aeruginosa trp cells with the RSF1010-trp hybrid plasmid and high levels of enzyme activity from the gene on the plasmid. Journal of Bacteriology 136:312–317
    [Google Scholar]
  14. Nagahari K., Tanaka T., Hishinuma F., Kuroda M., Sakaguchi K. 1977; Control of tryptophan synthetase amplified by varying the numbers of composite plasmids in Escherichia coli cells. Gene 1:141–152
    [Google Scholar]
  15. Nisen P. D., Kopecko D. J., Chou J., Cohen S. N. 1977; Site-specific DNA deletions occurring adjacent to the termini of a transposable ampicillin resistance element (Tn3). Journal of Molecular Biology 117:975–998
    [Google Scholar]
  16. Noel K. D., Ames G. F. L. 1978; Evidence for a common mechanism for the insertion of the Tn10 transposon and for the generation of Tn10-stimulated deletions. Molecular and General Genetics 166:217–223
    [Google Scholar]
  17. Ohtsubo H., Ohmori H., Ohtsubo E. 1979; Nucleotide-sequence analysis of Tn3 (Ap): implications for insertion and deletion. Cold Spring Harbor Symposia on Quantitative Biology 43:1269–1277
    [Google Scholar]
  18. Ptashne M., Hopkins N. 1968; The operators controlled by the λ phage repressor. Proceedings of the National Academy of Sciences of the United States of America 60:1282–1287
    [Google Scholar]
  19. Sakakibara Y., Tomizawa J. 1974; Replication of colicin E1 plasmid DNA in cell extracts. Proceedings of the National Academy of Sciences of the United States of America 71:802–806
    [Google Scholar]
  20. Smith D. H., Yanofsky C. 1962; Enzymes involved in the biosynthesis of tryptophan. Methods in Enzymology 5:794–806
    [Google Scholar]
  21. Smith D. L., Blattner F. R., Davies J. 1976; The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii. . Nucleic Acids Research 3:343–353
    [Google Scholar]
  22. So M., Gill R., Falkow S. 1975; The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA. Molecular and General Genetics 142:239–249
    [Google Scholar]
  23. Takeya T., Nomiyama H., Miyoshi J., Shimada K., Takagi Y. 1979; DNA sequences of the integration sites and inverted repeated structure of transposon Tn3. Nucleic Acids Research 6:1831–1841
    [Google Scholar]
  24. Tanaka T., Weisblum B. 1975; Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. Journal of Bacteriology 121:345–362
    [Google Scholar]
  25. Tu C.-P.D., Cohen S. N. 1980; Effect of DNA sequences adjacent to the termini of Tn3 on sequential translocation. Molecular and General Genetics 177:597–601
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-127-1-93
Loading
/content/journal/micro/10.1099/00221287-127-1-93
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error