1887

Abstract

A mating system to transfer the ability of growing autotrophically as a hydrogen bacterium ( marker) has been developed in strain 1b. Following filter mating between an strain and an strain, transconjugants of the phenotype AutTrpStr were obtained. The number of recombinant colonies exceeded the number of revertants by a factor of 10. This and other crosses indicated that only the marker is transferred. Among other markers tested (growth on lactate and benzoate) no new combinations were found. A few strains of the were tested for their ability to act as recipients. Transconjugants of and able to grow autotrophically were isolated. Most transconjugants were able to act as donors, but with different recipient spectra. As indicated by the observation of sectored colonies after autotrophic growth, by the measurement of the frequency of loss of autotrophy compared to auxotrophic markers, and by the effect of mitomycin C treatment, the marker does not become integrated in the recipient chromosome in a stable state. It is probably located on a plasmid.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-126-2-327
1981-10-01
2021-07-31
Loading full text...

Full text loading...

/deliver/fulltext/micro/126/2/mic-126-2-327.html?itemId=/content/journal/micro/10.1099/00221287-126-2-327&mimeType=html&fmt=ahah

References

  1. Abdelal A. T., Schlegel H. G. 1974; Purification and regulatory properties of fructose 1,6-diphosphatase from Hydrogenomonas eutropha. Journal of Bacteriology 120:304–310
    [Google Scholar]
  2. Adams J. N., Bradley S. G. 1963; Recombination events in the bacterial genus Nocardia. Science 140:1392–1394
    [Google Scholar]
  3. Aggag M., Schlegel H. G. 1973; Studies on a Gram-positive hydrogen bacterium, Nocardia opaca strain lb. I. Description and physiological characterization. Archiv für Mikrobiologie 88:299–318
    [Google Scholar]
  4. Aggag M., Schlegel H. G. 1974; Studies on a Gram-positive hydrogen bacterium, Nocardia opaca lb. III.Purification, stability and some properties of the soluble hydrogen dehydrogenase. Archives of Microbiology 100:25–39
    [Google Scholar]
  5. Amachi T., Bowien B. 1979; Characterization of two fructose bisphosphatase isoenzymes from the hydrogen bacterium Nocardia opaca lb. Journal of General Microbiology 113:347–356
    [Google Scholar]
  6. Andersen K. 1979; Mutations altering the catalytic activity of a plant-type ribulosebisphosphate carboxylase/oxygenase in Alcaligenes eutrophus. Biochimica et biophysica acta 585:1–11
    [Google Scholar]
  7. Bowien B., Schlegel H. G. 1981; Physiology and biochemistry of aerobic hydrogen-oxidizing bacteria. Annual Review of Microbiology 35: (in the Press)
    [Google Scholar]
  8. Brewin N. J., De Jong T. M., Phillips D. A., Johnston A. W. B. 1980; Co-transfer of determinants for hydrogenase activity and nodulation ability in Rhizobium leguminosarum. Nature; London: 28877–79
    [Google Scholar]
  9. Brownell G. H. 1978; Genetic interactions in the genus Nocardia. In Genetics of the Actinomycetales pp. 137–148 Edited by Freerksen E., Tarnok I., Thumin J. H. Stuttgart & New York: Gustav Fischer Verlag;
    [Google Scholar]
  10. Brownell G. H., Adams J. N., Bradley S. G. 1967; Growth and characterization of nocardiophages for Nocardia canicruria and Nocardia erythropolis mating types. Journal of General Microbiology 47:247–256
    [Google Scholar]
  11. Friedrich B., Hogrefe C., Schlegel H. G. 1981; Naturally occurring genetic transfer of hydrogen-oxidizing ability between strains of Alcaligeneseutrophus. Journal of Bacteriology 147:198–205
    [Google Scholar]
  12. Goodfellow M. G., Alderson G. 1977; The actinomycete genus Rhodococcus: a home for the ‘rhodochrous’ complex. Journal of General Microbiology 100:99–122
    [Google Scholar]
  13. Matney T. S., Achenbach N. E. 1962; A comment on the fertility of F2 donor types of Escherichia coli K-12. Biochemical and Biophysical Research Communications 9:285–287
    [Google Scholar]
  14. Mizuguchi Y., Suga K., Tokunaga T. 1976; Multiple mating types of Mycobacterium smegmatis. Japanese Journal of Microbiology 20:435–443
    [Google Scholar]
  15. Pootjes C. F. 1977; Evidence for plasmid coding of the ability to utilize hydrogen gas by Pseudomonas facilis. Biochemical and Biophysical Research Communications 76:1002–1006
    [Google Scholar]
  16. Reh M., Schlegel H. G. 1975; Chemolithoautotrophie als eine übertragbare, autonome Eigenschaft von Nocardia opaca lb. Nachrichten der Akademie der Wissenschaften Göttingen 12:1–10
    [Google Scholar]
  17. Schink B., Schlegel H. G. 1978; Mutants of Alcaligen eseutrophus defective in autotrophic metabolism. Archives of Microbiology 117:123–129
    [Google Scholar]
  18. Schlegel H. G., Kaltwasser H., Gottschalk G. 1961; Ein Submersverfahrenzur Kultur wasser-stoffoxidierender Bakterien:wachstumsphysiologische Untersuchungen. Archiv für Mikrobiologie 38:209–222
    [Google Scholar]
  19. Schneider K., Schlegel H. G. 1977; Localization and stability of hydrogenases from aerobic hydrogen bacteria. Archives of Microbiology 112:229–238
    [Google Scholar]
  20. Watanabe T. 1963; Infective heredity of multiple drug resistance in bacteria. Bacteriological Reviews 27:87–115
    [Google Scholar]
  21. Wheelis M. L. 1975; The genetics of dissimilatory pathways in Pseudomonas. Annual Review of Microbiology 29:505–524
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-126-2-327
Loading
/content/journal/micro/10.1099/00221287-126-2-327
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error