1887

Abstract

Summary: Pea plants that were nodulated by strains 248, 300 or 3622 and grown in the absence of combined nitrogen differed significantly in dry weight, leaf area, nitrogen content, nodule mass and nodule number. Transfer of plasmids from these field isolates to strain 16015, a non-nodulating derivative of strain 300, resulted in new strains capable of both nodulation and N fixation. In plants nodulated by these new strains the numbers of root nodules were significantly different, but there were no significant differences in N fixation, leaf area or dry weight. In several cases the introduction of additional plasmids into strain 300 or strain 16015 impaired symbiotic performance relative to strain 300 itself. Of all plant traits measured in symbiotic associations, leaf area was most highly correlated with the total Kjeldahl nitrogen content of plants after 25 d growth in the absence of combined nitrogen.

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1981-05-01
2021-10-24
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References

  1. Berg D.E., Davies J., Allet B., Rochaix J. 1975; Transposition of R factor genes to bacteriophage λ. Proceedings of the National Academy of Sciences of the United States of America 72:3628–3632
    [Google Scholar]
  2. Beringer J.E. 1974; R factor transfer in Rhizobium leguminosarum. Journal of General Microbiology 84:188–198
    [Google Scholar]
  3. Beringer J.E., Hoggan S.A., Johnston A.W.B. 1978; Linkage mapping in Rhizobium leguminosarum by means of R plasmid-mediated recombination. Journal of General Microbiology 104:201–207
    [Google Scholar]
  4. Bethlenfalvay G.J., Phillips D.A. 1977; Ontogenetic interactions between photosynthesis and symbiotic nitrogen fixation in legumes. Plant Physiology 60:419–421
    [Google Scholar]
  5. Bethlenfalvay G.J., Phillips D.A. 1979; Variation in nitrogenase and hydrogenase activity of Alaska pea root nodules. Plant Physiology 63:816–820
    [Google Scholar]
  6. Bethlenfalvay G.J., Abu-Shakra S.S., Phillips D.A. 1978; Interdependence of nitrogen nutrition and photosynthesis in Pisum sativum L. II.Host plant response to nitrogen fixation by Rhizobium strains. Plant Physiology 62:131–133
    [Google Scholar]
  7. Brewin N.J., Beringer J.E., Buchanan-Wollaston A.V., Johnston A.W.B., Hirsch P.R. 1980a; Transfer of symbiotic genes with bacteriocinogenic plasmids in Rhizobium leguminosarum. Journal of General Microbiology 116:261–270
    [Google Scholar]
  8. Brewin N.J., Beringer J.E., Johnston A.W.B. 1980b; Plasmid-mediated transfer of host range specificity between two strains of Rhizobium leguminosarum. Journal of General Microbiology 120:413–420
    [Google Scholar]
  9. Bromfield E.S.P., Jones D.G. 1979; The competitive ability and symbiotic effectiveness of doubly labelled antibiotic resistant mutants of Rhizobium trifolii. Annals of Applied Biology 91:211–219
    [Google Scholar]
  10. Buchanan-Wollaston A.V., Beringer J.E., Brewin N.J., Hirsch P.R., Johnston A.W.B. 1980; Identification of symbiotically defective mutants by insertion of the transposon Tn5 into a transmissible plasmid. Molecular and General Genetics 178:185–190
    [Google Scholar]
  11. Burns R.C., Hardy R.W.F. 1975 Nitrogen Fixation in Bacteria and Higher Plants pp. 121–122 New York, Heidelberg & Berlin:: Springer-Verlag.;
    [Google Scholar]
  12. Burris R.H., Wilson P.W. 1957; Methods for measurement of nitrogen fixation. Methods in Enzymology 4:355–366
    [Google Scholar]
  13. Dunican L.K., O’Gara F., Tierney A.B. 1976; Plasmid control of effectiveness in Rhizobium: transfer of nitrogen-fixing genes on a plasmid from Rhizobium trifolii to Klebsiella aerogenes. In Symbiotic Nitrogen Fixation in Plants pp. 77–90 Nutman P.S. Edited by Cambridge:: Cambridge University Press.;
    [Google Scholar]
  14. Hagedorn C. 1979; Relationship of antibiotic resistance to effectiveness in Rhizobium trifolii populations. Soil Science Society of America Journal 43:921–925
    [Google Scholar]
  15. Higashi S. 1967; Transfer of clover infectivity of Rhizobium trifolii to Rhizobium phaseoli as mediated by an episomic factor. Journal of General and Applied Microbiology 13:391–403
    [Google Scholar]
  16. Hirsch P.R. 1979; Plasmid-determined bacteriocin production by Rhizobium leguminosarum. Journal of General Microbiology 113:219–228
    [Google Scholar]
  17. Hirsch P.R., Van Montagu M., Johnston A.W.B., Brewin N.J., Schell J. 1980; Physical identification of bacteriocinogenic, nodulation and other plasmids in strains of Rhizobium leguminosarum. Journal of General Microbiology 120:403–412
    [Google Scholar]
  18. Johnson C.M., Stout P.R., Broyer T.C., Carlton A.B. 1957; Comparative chlorine requirements of different plant species. Plant and Soil 8:337–353
    [Google Scholar]
  19. Johnston A.W.B., Beringer J.E. 1975; Identification of the Rhizobium strains in pea root nodules using genetic markers. Journal of General Microbiology 87:343–350
    [Google Scholar]
  20. Johnston A.W.B., Beynon J.L., Buchanan-Wollaston A.V., Setchell S.M., Hirsch P.R., Beringer J.E. 1978; High frequency transfer of nodulating ability between strains and species of Rhizobium. Nature; London: 276635–636
    [Google Scholar]
  21. Josey D.P., Beynon J.L., Johnston A.W.B., Beringer J.E. 1979; Strain identification in Rhizobium using intrinsic antibiotic resistance. Journal of Applied Bacteriology 46:343–350
    [Google Scholar]
  22. Leonard L.T. 1943; A simple assembly for use in testing of cultures of rhizobia. Journal of Bacteriology 45:523–527
    [Google Scholar]
  23. Nuti M.P., Lepidi A.A., Prakash R.K., Schilperoort R.A., Cannon F.C. 1979; Evidence for nitrogen fixation (nif) genes on indigenous Rhizobium plasmids. Nature; London: 282533–535
    [Google Scholar]
  24. Pankhurst C.E. 1977; Symbiotic effectiveness of antibiotic-resistant mutants of fast- and slow- growing strains of Rhizobium nodulatingLotus species. Canadian Journal of Microbiology 23:1026–1033
    [Google Scholar]
  25. Pate J.S., Layzell D.B., Atkins C.A. 1979; Economy of carbon and nitrogen in a nodulated and nonnodulated (NO3-grown) legume. Plant Physiology 64:1083–1088
    [Google Scholar]
  26. Schwinghamer E.A. 1964; Association between antibiotic resistance and ineffectiveness in mutant strains of Rhizobium spp. Canadian Journal of Microbiology 10:221–233
    [Google Scholar]
  27. Schwinghamer E.A. 1967; Effectiveness of Rhizobium as modified by mutation for resistance to antibiotics. Antonie van Leeuwenhoek 33:122–136
    [Google Scholar]
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