A study of the nutritional requirements of showed that this parasitic fungus grew well in a medium containing: KHPO, 0·004; MgSO.7HO, 0·002; NHNO, 0·0375; glucose 4% (w/v); Zn and Mn 2 p.p.m.; thiamine, pyridoxine, nicotinic acid, folic acid, ascorbic acid. Without thiamine practically no growth of the fungus took place; omission of pyridoxine had nearly as adverse an effect. Raffinose and cellobiose were the best carbon sources; glucose also gave a good yield of mycelium. Statistical analysis of factorial experiments indicated that a correct balance between the mineral constituents of the medium and between the carbon and nitrogen sources is essential in order to obtain a high yield of mycelium.


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