1887

Abstract

Fluctuations in glutamine synthetase activity were observed when exponentially dividing cells of were synchronized by continuous-flow centrifugation. These fluctuations were not seen in a control culture in which cells were allowed to grow asynchronously following filtration. However, when an asynchronous control culture was prepared by allowing cells to pass quickly through the continuous-action rotor without size selection, marked fluctuations in glutamine synthetase activity occurred. It is concluded that when the continuous-flow size selection method of cell synchronization is used, suitable control experiments are essential before any conclusions can be drawn about how enzyme synthesis is regulated during the cell cycle.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-119-2-543
1980-08-01
2021-05-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/119/2/mic-119-2-543.html?itemId=/content/journal/micro/10.1099/00221287-119-2-543&mimeType=html&fmt=ahah

References

  1. Duffus J. H., Finbow M. E., Paterson L. J., Slaughter J. C. 1974; Glutamine amido transferase in yeast: changes in activity during the cell cycle.. Journal of General Microbiology 83:183–186
    [Google Scholar]
  2. Eaton N. R. 1962; New press for disruption of microorganisms.. Journal of Bacteriology 83:1359–1360
    [Google Scholar]
  3. Evans J. B. 1975; Preparation of synchronous cultures of Escherichia coli by continuous-flow size selection.. Journal of General Microbiology 91:188–191
    [Google Scholar]
  4. Folkes B. F., Bishop R., Nurse P. M., Sims A. P. 1973; Some aspects of the regulation of amino acid metabolism during the cell cycle of the yeast, Candida utilis.. Proceedings of the Second European Symposium on the Cell Cycle, Veröffentlichungen der Universität Innsbruck 7722–25
    [Google Scholar]
  5. Lloyd D., John L., Edwards C., Chagla A. H. 1975; Synchronous cultures of micro-organisms: large-scale preparation by continuous-flow size selection.. Journal of General Microbiology 88:153–158
    [Google Scholar]
  6. Lloyd D., John L., Hamill M., Phillips C. A., Kader J., Edwards S. W. 1977; Continuousflow cell cycle fractionation of eukaryotic microorganisms.. Journal of General Microbiology 99:223–227
    [Google Scholar]
  7. Mitchison J. M. 1970; Physiological and cytological methods for Schizosaccharomyces pombe.. Methods in Cell Physiology 4:131–165
    [Google Scholar]
  8. Mitchison J. M. 1977; Enzyme synthesis during the cell cycle.. In Cell Differentiation in Microorganisms, Plants and Animals pp. 377–401 Edited by Nover L., Mothes K. Jena: Gustav Fischer Verlag;
    [Google Scholar]
  9. Phillips C. A., Lloyd D. 1978; Continuousflow size selection of Tetrahymena pyriformis ST: changes in volume, DNA, RNA and protein during synchronous growth.. Journal of General Microbiology 105:95–103
    [Google Scholar]
  10. Poole R. K. 1977; Preparation of synchronous cultures of microorganisms by continuous-flow selection: which cells are selected ?. FEMS Microbiology Letters 1:305–307
    [Google Scholar]
  11. Shapiro B. M., Stadtman E. R. 1970; Glutamine synthetase (Escherichia coll).. Methods in Enzymology 17A:910–922
    [Google Scholar]
  12. Walker G. M., Duffus J. H. 1979; An investigation into the potential use of chelating agents and antibiotics as synchrony inducers in the fission yeast Schizosaccharomyces pombe.. Journal of General Microbiology 114:391–400
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-119-2-543
Loading
/content/journal/micro/10.1099/00221287-119-2-543
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error