1887

Abstract

Summary: Fluctuations in glutamine synthetase activity were observed when exponentially dividing cells of were synchronized by continuous-flow centrifugation. These fluctuations were not seen in a control culture in which cells were allowed to grow asynchronously following filtration. However, when an asynchronous control culture was prepared by allowing cells to pass quickly through the continuous-action rotor without size selection, marked fluctuations in glutamine synthetase activity occurred. It is concluded that when the continuous-flow size selection method of cell synchronization is used, suitable control experiments are essential before any conclusions can be drawn about how enzyme synthesis is regulated during the cell cycle.

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/content/journal/micro/10.1099/00221287-119-2-543
1980-08-01
2019-10-23
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-119-2-543
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