1887

Abstract

To detect the effect of the postulated inducible error-prone repair system (‘SOS repair’) on the bacterial chromosome, an Hfr strain JC5088 was u.v.-irradiated immediately before mating it with recipients in which SOS repair was supposed to be functioning either through expression, u.v. irradiation or the presence of plasmid pKM101. The recombinant yields of these crosses were compared with those obtained in corresponding crosses with recipients in which SOS repair either was not induced or was totally eliminated by the mutation. No difference in marker recovery efficiency could be detected between these two sets of recipients and thus no induced repair process acting on donor DNA could be demonstrated. The possible reasons for this finding are discussed.

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/content/journal/micro/10.1099/00221287-119-2-327
1980-08-01
2024-12-12
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References

  1. Castellazzi M., George J., Buttin G. 1972; Prophage induction and cell division in E. coli. I. Further characterization of the thermosensitive mutation tif-1 whose expression mimics the effect of UV-irradiation.. Molecular and General Genetics 119:139–152
    [Google Scholar]
  2. Castellazzi M., George J., Buttin G. 1973; Prophage induction and cell division in E. coli. II. Linked (recA zab) and unlinked (lex) suppressors of tif-mediated induction and filamentation.. Molecular and General Genetics 119:153–174
    [Google Scholar]
  3. Davis B. D., Mingioli E. S. 1950; Mutants of Escherichia coli requiring methionine or vitamin B12.. Journal of Bacteriology 60:17–28
    [Google Scholar]
  4. Defais M., Fauquet P., Radman M., Errera M. 1971; Ultraviolet reactivation and ultraviolet mutagenesis of lambda in different genetic systems.. Virology 43:495–503
    [Google Scholar]
  5. Donch J., Green M. H. L., Greenberg J. 1968; Interaction of the exr and Ion genes in Escherichia coli.. Journal of Bacteriology 96:1704–1710
    [Google Scholar]
  6. Doubleday O. P., Green M. H. L., Bridges B. A. 1977; Spontaneous and ultraviolet-induced mutation in Escherichia coli: interaction between plasmid and tif-1 mutator effects.. Journal of General Microbiology 101:163–166
    [Google Scholar]
  7. Green M. H. L., Bridges B. A., Riazuddin S. 1971; Effect of Ɣ-radiation on the donor ability of recA and recA+ strains of Escherichia coli.. Journal of General Microbiology 67:
    [Google Scholar]
  8. Howard-Flanders P., Boyce R. P., Theriot L. 1966; Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and other mutagen products from DNA.. Genetics 53:1119–1136
    [Google Scholar]
  9. Howard-Flanders P., Rupp W. D., Wilkins B. M., Cole R. S. 1968; DNA replication and recombination after UV irradiation.. Cold Spring Harbor Symposia on Quantitative Biology 33:195–205
    [Google Scholar]
  10. Kirby E. P., Jacob F., Goldthwait D. A. 1967; Prophage induction and filament formation in a mutant strain of Escherichia coli.. Proceedings of the National Academy of Sciences of the United States of America 58:1903
    [Google Scholar]
  11. Lloyd R. G. 1978; Hyper-recombination in Escherichia coli K-12 mutants constitutive for protein X synthesis.. Journal of Bacteriology 134:929–935
    [Google Scholar]
  12. Mortelmans K. E., Stocker B. A. D. 1976; Ultraviolet light protection, enhancement of ultraviolet light mutagenesis, and mutator effect of plasmid R46 in Salmonella typhimurium.. Journal of Bacteriology 128:271–282
    [Google Scholar]
  13. Ono J., Shimazu Y. 1966; Ultraviolet reactivation of a bacteriophage containing a singlestranded deoxyribonucleic acid as A genetic element.. Virology 29:295–301
    [Google Scholar]
  14. Radman M. 1974; Phenomenology of AN inducible mutagenic DNA repair pathway in Escherichia coli: SOS repair hypothesis. . In Molecular and Environmental Aspects of Mutagenesis pp. 355–367 Prakash L., Sherman F., Miller M. W., Lawrence C. W., Taber H. W. Edited by Springfield, Illinois: C. C. Thomas;
    [Google Scholar]
  15. Radman M. 1975; SOS-repair hypothesis: Phenomenology of an inducible DNA repair which is accompanied by mutagenesis.. In Molecular Mechanisms for Repair of DNA pp. 355–367 Hanawalt P. C., Setlow R. B. Edited by New York: Plenum Press;
    [Google Scholar]
  16. Rupp W. D., Howard-Flanders P. 1968; Discontinuities in the DNA synthesized in an excision-defective strain of Escherichia coli following ultraviolet irradiation.. Journal of Molecular Biology 31:291–304
    [Google Scholar]
  17. Walker G. C. 1977; Plasmid (pKM101) mediated enhancement of repair and mutagenesis: dependence on chromosomal genes in Escherichia coli K-12.. Molecular and General Genetics 152:93–103
    [Google Scholar]
  18. Weigle J. J. 1953; Induction of mutation in a bacterial virus.. Proceedings of the National Academy of Sciences of the United States of America 39:628–636
    [Google Scholar]
  19. Witkin E. M. 1974; Thermal enhancement of ultraviolet mutability in a tif-1 uvrA derivative of Escherichia coli B/r: evidence that ultraviolet mutagenesis depends upon an inducible function.. Proceedings of the National Academy of Sciences of the United States of America 71:1930–1934
    [Google Scholar]
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