1887

Abstract

strain PC-7 carries two independent temperature-sensitive mutations, one affecting the restriction and modification (R-M) phenotype and the other the DnaC(D) phenotype. The results of complementation and P1 transduction analysis of the mutation affecting the R-M phenotype implicate a fourth gene, designated , located close to the three-gene complex. The properties of merodiploids constructed between appropriate recipients and F' elements with different mutations in , and genes might indicate that in strain PC-7 the temperature-sensitive products, determined by and cistrons, are synthesized. The role of the temperature-sensitive () gene product in the formation of the restriction endonuclease was studied and no direct relation was found between the DnaC(D) and R-M phenotypes.

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/content/journal/micro/10.1099/00221287-119-1-231
1980-07-01
2024-11-03
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References

  1. Appleyard R. K. 1954; Segregation of new lysogenic types during growth of doubly lysogenic strains derived from Escherichia coli K-12.. Genetics 39:440–452
    [Google Scholar]
  2. Arber W., Dussoix D. 1962; Host specificity of DNA produced by Escherichia coli. I. Host controlled modification of bacteriophage λ.. Journal of Molecular Biology 5:18–36
    [Google Scholar]
  3. Arber W., Linn S. 1969; DNA modification and restriction.. Annual Review of Biochemistry 38:467–500
    [Google Scholar]
  4. Bertani G., Weigle J. J. 1953; Host controlled variation in bacterial viruses.. Journal of Bacteriology 65:113–121
    [Google Scholar]
  5. Boyer H. W., Roulland-Dussoix D. 1969; A complementation analysis of the restriction and modification of DNA in Escherichia coli.. Journal of Molecular Biology 41:459–472
    [Google Scholar]
  6. Eskin B., Linn S. 1972; The deoxyribonucleic acid modification and restriction enzymes of Escherichia coli B. II.Purification, subunit structure and catalytic properties of the restriction endonuclease.. Journal of Biological Chemistry 247:6183–6191
    [Google Scholar]
  7. Glover S. W. 1970; Functional analysis of host-specificity mutants in Escherichia coli.. Genetical Research 15:237–250
    [Google Scholar]
  8. Glover S. W., Colson C. 1969; Genetics of host-controlled restriction and modification in Escherichia coli.. Genetical Research 13:227–240
    [Google Scholar]
  9. Howard-Flanders P., Theriot L. 1966; Mutations of Escherichia coli K12 defective in DNA repair and genetic recombination.. Genetics 53:1137–1150
    [Google Scholar]
  10. Hubáček J. 1973; Functional analysis of second-step host specificity mutations in unstable Escherichia coli heterozygotes.. Journal of General Microbiology 79:257–264
    [Google Scholar]
  11. Hubáček J., Glover S. W. 1970; Complementation analysis of temperature-sensitive host specificity mutations in Escherichia coli.. Journal of Molecular Biology 50:111–127
    [Google Scholar]
  12. Low B. 1968; Formation of merodiploids in matings with a class of rec recipient strains of Escherichia coli K12.. Proceedings of the National Academy of Sciences of the United States of America 60:160–167
    [Google Scholar]
  13. Paul A. V., Inouye M. 1974; Temperature-sensitive modification and restriction phenotypes of an Escherichia coli dnaD mutant.. Journal of Bacteriology 119:907–912
    [Google Scholar]
  14. Rosner J. L. 1972; Formation, induction and curing of bacteriophage PI lysogens.. Virology 48:679–689
    [Google Scholar]
  15. Wechsler J. A. 1975; Genetic and phenotypic characterization of dnaC mutations.. Journal of Bacteriology 121:594–599
    [Google Scholar]
  16. Willetts N. S., Clark A. J. 1969; Characteristics of some multiple recombination-deficient strains of Escherichia coli.. Journal of Bacteriology 100:231–239
    [Google Scholar]
  17. Yuan R., Bickle T. A., Ebbers W., Brack CH. 1975; Multiple steps in DNA recognition by restriction endonuclease from E. coli K.. Nature,London 256:556–560
    [Google Scholar]
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