%0 Journal Article %A Hubáček, Josef %A Weiserová, Marie %T DNA Restriction and Modification in Escherichia coli: Functional Analysis of the Role of the dnaC(D) Gene Product %D 1980 %J Microbiology, %V 119 %N 1 %P 231-238 %@ 1465-2080 %R https://doi.org/10.1099/00221287-119-1-231 %I Microbiology Society, %X Escherichia coli strain PC-7 carries two independent temperature-sensitive mutations, one affecting the restriction and modification (R-M) phenotype and the other the DnaC(D) phenotype. The results of complementation and P1 transduction analysis of the mutation affecting the R-M phenotype implicate a fourth gene, designated hsdX, located close to the hsd three-gene complex. The properties of merodiploids constructed between appropriate recipients and F' elements with different mutations in hsdS, hsdR and hsdM genes might indicate that in strain PC-7 the temperature-sensitive products, determined by hsdR and hsdS K cistrons, are synthesized. The role of the temperature-sensitive dnaC(D) gene product in the formation of the restriction endonuclease was studied and no direct relation was found between the DnaC(D) and R-M phenotypes. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-119-1-231