1887

Abstract

The regulation of the catabolic pathway for the degradation of naphthalene specified by the plasmids NAH, pND140 and pND160 was studied using plasmid-borne regulatory mutants in a host strain. Growth of strains harbouring the parent plasmids in the presence of salicylate resulted in induction of selected enzymes involved in the conversion of naphthalene to catechol (naphthalene oxygenase, salicylaldehyde dehydrogenase and salicylate hydroxylase) and enzymes of the -cleavage pathway (catechol 2,3-dioxygenase, 2-hydroxymuconic semialdehyde hydrolase and 2-hydroxymuconic semi-aldehyde dehydrogenase). Partial induction was also observed for all the NAH-encoded enzymes assayed when using -toluate as the inducing compound, over a genetic block. Mutants were obtained for each plasmid where the three enzymes of the -cleavage pathway were produced constitutively suggesting that the enzymes of the cleavage pathway belong to one operon. In these mutants, enzymes involved in the conversion of naphthalene to catechol were not produced constitutively but remained inducible during growth on salicylate indicating that these enzymes belong to a separate operon or operons.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-117-2-521
1980-04-01
2021-05-11
Loading full text...

Full text loading...

/deliver/fulltext/micro/117/2/mic-117-2-521.html?itemId=/content/journal/micro/10.1099/00221287-117-2-521&mimeType=html&fmt=ahah

References

  1. Austen R. A., Dunn N. W. 1977a; A comparative study of the NAH and TOL catabolic plasmids in Pseudomonasputida.. Australian Journal of Biological Sciences 30:357–366
    [Google Scholar]
  2. Austen R. A., Dunn N. W. 1977b; Isolation of mutants with altered metabolic control of the NAH plasmid-encoded catechol meta cleavage pathway.. Australian Journal of Biological Sciences 30:583–592
    [Google Scholar]
  3. Barnsley E. A. 1975; The induction of the enzymes of naphthalene metabolism in pseudomonads by salicylate and 2-aminobenzoate.. Journal of General Microbiology 88:193–196
    [Google Scholar]
  4. Barnsley E. A. 1976a; Role and regulation of the ortho and meta pathways of catechol metabolism in pseudomonads metabolising naphthalene and salicylate.. Journal of Bacteriology 125:404–408
    [Google Scholar]
  5. Barnsley E. A. 1976b; Naphthalene metabolism by pseudomonads: the oxidation of 1,2-dihydr- oxynaphthalene to 2-hydroxychromene-2-carbo- xylic acid and the formation of 2-hydroxybenzal- pyruvate.. Biochemical and Biophysical Research Communications 72:1116–1121
    [Google Scholar]
  6. Caterall F. A., Murray K., Williams P. A. 1971a; The configuration of the 1,2-dihydroxy- 1,2-dihydronaphthalene formed in the bacterial metabolism of naphthalene.. Biochimica et biophysica acta 237:361–364
    [Google Scholar]
  7. Caterall F. A., Sala-Trepat J. M., Williams P. A. 1971b; The coexistence of two pathways for the metabolism of 2-hydroxymuconic semialdehyde in a naphthalene-grown pseudomonad.. Biochemical and Biophysical Research Communications 43:463–469
    [Google Scholar]
  8. Chakrabarty A. M. 1972; Genetic basis of the biodegradation of salicylate in Pseudomonas.. Journal of Bacteriology 112:815–823
    [Google Scholar]
  9. Davies J. I., Evans W. C. 1964; Oxidative metabolism of naphthalene by soil pseudomonads.. Biochemical Journal 91:251–261
    [Google Scholar]
  10. Dunn N. W., Gunsalus I. C. 1973; Transmissible plasmid coding early enzymes of naphthalene oxidation in Pseudomonas putida.. Journal of Bacteriology 114:974–979
    [Google Scholar]
  11. Dunn N. W., Dunn H. M., Austen R. A. 1980; Evidence for the existence of two catabolic plasmids coding for the degradation of naph thalene.. Journal of General Microbiology 117:529–533
    [Google Scholar]
  12. Jerina D. M., Daly J. W., Jeffrey A. M., Gibson D. T. 1971; cis-1,2-Dihydroxy-1,2- dihydronaphthalene: a bacterial metabolite from naphthalene.. Archives of Biochemistry and Biophysics 142:394–396
    [Google Scholar]
  13. Murray K., Williams P. A. 1974; Role of catechol and the methylcatechols as inducers of aromatic metabolism in Pseudomonas putida.. Journal of Bacteriology 117:1153–1157
    [Google Scholar]
  14. Nozaki M., Kagamiyama H., Hayaishi O. 1963; Metapyrocatechase. I. Purification, crystallisation and some properties.. Biochemische Zeitschrift 338:582–586
    [Google Scholar]
  15. Sala-Trepat J. M., Murray K., Williams P. A. 1972; The metabolic divergence in the metacleavage of catechols by Pseudomonas putida NCIB 10015.. European Journal of Biochemistry 28:347–356
    [Google Scholar]
  16. Shamsuzzaman K. M., Barnsley E. A. 1974a; The regulation of naphthalene oxygenase in pseudomonads.. Journal of General Microbiology 83:165–170
    [Google Scholar]
  17. Shamsuzzaman K. M., Barnsley E. A. 1974b; The regulation of naphthalene metabolism in pseudomonads.. Biochemical and Biophysical Research Communications 60:582–589
    [Google Scholar]
  18. White G. P., Dunn N. W. 1977; Apparent fusion of the TOL plasmid with the R91 drug resistance plasmid in Pseudomonas aeruginosa.. Australian Journal of Biological Sciences 30:345–355
    [Google Scholar]
  19. Williams P. A., Murray K. 1974; Metabolism of benzoate and the methylbenzoates of Pseudomonas putida (arvilla) mt-2: evidence for the existence of the TOL plasmid.. Journal of Bacteriology 120:416–423
    [Google Scholar]
  20. Williams P. A., Catterall F. A., Murray K. 1975; Metabolism of naphthalene, 2-methyl- naphthalene, salicylate and benzoate by Pseudomonas PG: regulation of tangential pathways.. Journal of Bacteriology 124:680–685
    [Google Scholar]
  21. Wong C. L., Dunn N. W. 1974; Transmissible plasmid coding for the degradation of benzoate and m-toluate in Pseudomonas arvilla mt-2.. Genetical Research 23:227–232
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-117-2-521
Loading
/content/journal/micro/10.1099/00221287-117-2-521
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error