1887

Abstract

SUMMARY: The regulation of the catabolic pathway for the degradation of naphthalene specified by the plasmids NAH, pND140 and pND160 was studied using plasmid-borne regulatory mutants in a host strain. Growth of strains harbouring the parent plasmids in the presence of salicylate resulted in induction of selected enzymes involved in the conversion of naphthalene to catechol (naphthalene oxygenase, salicylaldehyde dehydrogenase and salicylate hydroxylase) and enzymes of the -cleavage pathway (catechol 2,3-dioxygenase, 2-hydroxymuconic semialdehyde hydrolase and 2-hydroxymuconic semi-aldehyde dehydrogenase). Partial induction was also observed for all the NAH-encoded enzymes assayed when using -toluate as the inducing compound, over a genetic block. Mutants were obtained for each plasmid where the three enzymes of the -cleavage pathway were produced constitutively suggesting that the enzymes of the cleavage pathway belong to one operon. In these mutants, enzymes involved in the conversion of naphthalene to catechol were not produced constitutively but remained inducible during growth on salicylate indicating that these enzymes belong to a separate operon or operons.

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/content/journal/micro/10.1099/00221287-117-2-521
1980-04-01
2019-10-14
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-117-2-521
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