RT Journal Article SR Electronic(1) A1 Austen, R. A. A1 Dunn, N. W.YR 1980 T1 Regulation of the Plasmid-specified Naphthalene Catabolic Pathway of Pseudomonas putida JF Microbiology, VO 117 IS 2 SP 521 OP 528 DO https://doi.org/10.1099/00221287-117-2-521 PB Microbiology Society, SN 1465-2080, AB The regulation of the catabolic pathway for the degradation of naphthalene specified by the plasmids NAH, pND140 and pND160 was studied using plasmid-borne regulatory mutants in a Pseudomonas putida host strain. Growth of strains harbouring the parent plasmids in the presence of salicylate resulted in induction of selected enzymes involved in the conversion of naphthalene to catechol (naphthalene oxygenase, salicylaldehyde dehydrogenase and salicylate hydroxylase) and enzymes of the meta-cleavage pathway (catechol 2,3-dioxygenase, 2-hydroxymuconic semialdehyde hydrolase and 2-hydroxymuconic semi-aldehyde dehydrogenase). Partial induction was also observed for all the NAH-encoded enzymes assayed when using m-toluate as the inducing compound, over a genetic block. Mutants were obtained for each plasmid where the three enzymes of the meta-cleavage pathway were produced constitutively suggesting that the enzymes of the meta-cleavage pathway belong to one operon. In these mutants, enzymes involved in the conversion of naphthalene to catechol were not produced constitutively but remained inducible during growth on salicylate indicating that these enzymes belong to a separate operon or operons., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-117-2-521