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Volume 117, Issue 2

The Mitochondrial Cytochromes of an Unusual Budding Yeast, : Spectral Characterization Exploiting Fourth-order Finite Difference Analysis Free

Abstract

Low-temperature difference spectra of gradient-purified mitochondria from revealed the presence of and -type cytochromes, spectrally similar to those of other yeasts. Fourth-order finite difference analysis resolved the broad a-band of -and -type cytochromes into eight peaks. Absorption maxima at about 539, 543·5 and 547·5 nm were attributed to one, or perhaps two, cytochrome(s) that are loosely bound to the mitochondrial membrane. Cytochrome was identified at 5505 to 5515 nm. Maxima at about 554, 556, 559 and 562 nm were attributed to three or four distinct b-type cytochromes on the basis of their differential reduction by NADH, dithionite, or ascorbate plus ,-,-tetramethyl--phenylenediamine in the absence or presence of antimycin. Difference spectra in the presence of CO or cyanide indicated the presence of cytochromes (600 nm) and (608 nm). Finite difference analysis of the cytochromes oxidase peak centred at 600 nm revealed two components; the major component at 600 nm was identified as cytochrome and the minor component at 605 nm as cytochrome A further CO-binding haemoprotein was tentatively identified as cytochrome

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© Society for General Microbiology 1980
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1980-04-01
2024-12-08
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References

  1. Barnett J. A., Ingram M. 1955; Technique in the study of yeast assimilation reactions.. Journal of Applied Bacteriology 18:131–148
     [Google Scholar]
  2. Butler W. L. 1979; Fourth derivative spectra.. Methods in Enzymology 56:501–515
     [Google Scholar]
  3. Butler W. L., Hopkins D. W. 1970a; Higher derivative analysis of complex absorption spectra.. Photochemistry and Photobiology 12:439–450
     [Google Scholar]
  4. Butler W. L., Hopkins D. W. 1970b; An analysis of fourth derivative spectra.. Photochemistry and Photobiology 12:451–456
     [Google Scholar]
  5. Castor L. N., Chance B. 1955; Photochemical action spectra of carbon monoxide-inhibited respiration.. Journal of Biological Chemistry 217:453–465
     [Google Scholar]
  6. Chance B. 1957; Techniques for the assay of the respiratory enzymes.. Methods in Enzymology 4:273–329
     [Google Scholar]
  7. Chance B., Keyhani E., Saronio C. 1978; Oxygen metabolism in yeast cells.. In Biochemistry and Genetics of Yeast pp. 17–48 Edited by Horecker B. L., Bacila M. New York: Academic Press;
     [Google Scholar]
  8. Claisse M. L., Pajot P. F. 1974; Presence of cytochrome c1in cytoplasmic ‘petite’ mutants of Saccharomyces cerevisiae.. European Journal of Biochemistry 49:49–59
     [Google Scholar]
  9. Daniel R. M. 1970; The electron transport system of Acetobacter suboxydans with particular reference to cytochrome o.. Biochimica et biophysica acta 216:328–341
     [Google Scholar]
  10. Davis K. A., Hatefi Y., Poff K. L., Butler W. L. 1972; The b-type cytochromes of beef heart mitochondria.. Biochemical and Biophysical Research Communications 46:1984–1990
     [Google Scholar]
  11. Edwards S. W., Chagla A. H., Griffiths A. J., Lloyd D. 1977; The cytochromes of Acanthamoeba castellanii.. Biochemical Journal 168:113–121
     [Google Scholar]
  12. Fell J. W. 1966; Sterigmatomyces, a new fungal genus from marine areas.. Antonie van Leeuwenhoek 32:99–104
     [Google Scholar]
  13. Grimmelikhuijzen C. J. P., Marres C. A. M., Slater E. C. 1975; Antimycin-insensitive mutants of Candida utilis.II. The effects of antimycin on cytochrome 6.. Biochimica et biophysica acta 376:533–548
     [Google Scholar]
  14. Von Jagow G., Weiss H., Klingenberg M. 1973; Comparison of the respiratory chain of Neurospora crassa wild-type and the mi-mutants mi-1 and mi-3.. European Journal of Biochemistry 33:140–157
     [Google Scholar]
  15. Kuschmitz D., Hess B. 1975; Identification of cytochrome a and a3 in yeast cells.. Hoppe-Seyler’s Zeitschrift für physiologische Chemie 356:1139–1149
     [Google Scholar]
  16. Lang B., Burger G., Doxiadis I., Thomas D. Y., Bandlow W., Kaudewitz F. 1977; A simple method for the large-scale preparation of mitochondria from microorganisms.. Analytical Biochemistry 77:110–121
     [Google Scholar]
  17. Lemberg R., Barrett J. 1973; Physicochemical properties of cytochrome c.. In The Cytochromes pp. 170–177 London & New York: Academic Press;
     [Google Scholar]
  18. Lloyd D. 1974; Respiration and the respiratory components in mitochondria of micro-organisms.. In The Mitochondria of Micro-organisms pp. 82–158 London & New York: Academic Press;
     [Google Scholar]
  19. Mitsushima K., Shinmyo A., Enatsu T. 1977; Spectral properties of cytochromes in Candida lipolytica mitochondria.. Journal of Fermentation Technology 55:84–88
     [Google Scholar]
  20. Mok T. C. K., Rickard P. A. D., Moss F. J. 1969; The carbon monoxide-reactive haemoproteins of yeast.. Biochimica et biophysica acta 172:438–449
     [Google Scholar]
  21. Morrey J. R. 1968; On determining spectral peak positions from composite spectra with a digital computer.. Analytical Chemistry 40:905–914
     [Google Scholar]
  22. Oshino R., Oshino N., Chance B., Hagihara B. 1973; Studies on yeast hemoglobin.The properties of yeast hemoglobin and its physiological function in the cell.. European Journal of Biochemistry 35:23–33
     [Google Scholar]
  23. Poole R. K., Lloyd D., Chance B. 1974; The development of cytochromes during the cell cycle of a glucose-repressed fission yeast Schizosaccharomyces pombe 972h−.. Biochemical Journal 138:201–210
     [Google Scholar]
  24. Salmon I., Poole R. K. 1978a; Growth and cell division of a novel budding yeast, Sterigmatomyces halophilus.. Bulletin of the British Mycological Society 12:121
     [Google Scholar]
  25. Salmon I., Poole R. K. 1978b; The complexity of cytochromes in a budding yeast, Sterigmatomyces halophilus.. Society for General Microbiology Quarterly 6:34–35
     [Google Scholar]
  26. Sato N., Ohnishi T., Chance B. 1972; Spectral studies of 6 cytochromes in yeast mitochondria and intact cells.. Biochimica et biophysica acta 275:288–297
     [Google Scholar]
  27. Shipp W. S. 1971; Multiple b cytochromes in rat and pigeon mitochondria detected by finite difference analysis of absorption spectra.. Biochemical and Biophysical Research Communications 45:1437–1443
     [Google Scholar]
  28. Shipp W. S. 1972; Cytochromes of Escherichia coli.. Archives of Biochemistry and Biophysics 150:459–472
     [Google Scholar]
  29. Talsky G., Mayring L. 1978; On the analogous on-line derivation of higher order for the fine-resolving of UV-visible spectra and other electric signals.. Fresenius’ Zeitschrift für analytische Chemie 292:233–235
     [Google Scholar]
  30. Yonetani T. 1960; Studies on cytochrome oxidase. 1. Absolute and difference absorption spectra.. Journal of Biological Chemistry 235:845–852
     [Google Scholar]
  31. Yu C. A., Yu L., King T. E. 1972; Spectral evidence of multiple cytochromes b present in succinate: cytochrome c reductase.. Biochimica et biophysica acta 267:300–308
     [Google Scholar]
/content/journal/micro/10.1099/00221287-117-2-315
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The Mitochondrial Cytochromes of an Unusual Budding Yeast, Sterigmatomyces halophilus: Spectral Characterization Exploiting Fourth-order Finite Difference Analysis
Microbiology 117, 315 (1980); https://doi.org/10.1099/00221287-117-2-315
/content/journal/micro/10.1099/00221287-117-2-315
/content/journal/micro/10.1099/00221287-117-2-315
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