SUMMARY: A study was made of the morphology and growth of young mycelia of a temperature-sensitive mutant () of At 25 C the mutant grew at the same rate as the parental strain, formed septa and branched normally. At 30 and 37 C the mutant grew at about three-quarters of the rate of the parental strain and, unlike the parental strain, branched dichotomously. The mutant was aseptate at 37 C, and at 30 C produced fewer septa than the parental strain. No septal initials were detected in mutant mycelia grown at 37 C. Aseptate mycelia of the mutant were grown at 37 C and then transferred to 25 C. After transfer to 25 C, septa appeared parasynchronously throughout aseptate regions of the mycelium previously formed at 37 C. The intercalary compartments formed in this manner had a mean length which was identical with those of intercalary compartments formed by the parental strain at 37 C or the mutant at 25 C. The lag between transfer of mutant mycelia from 37 to 25 C and the first appearance of septa varied from 0.15 to 2.75 h; the maximum lag observed was similar to the organisms's doubling time (2.37 h) at 25 C.


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