1887

Abstract

The growth inhibition of mutants by sodium azide is reversed by cystine and related compounds. NADPH-sulphite reductase (hydrogen-sulphide: NADP oxidoreductase; EC 1.8.1.2), an enzyme of cysteine biosynthesis, is inhibited in cell extracts by sodium azide. AziB mutants which are able to grow in the presence of the inhibitor without cystine were isolated. About half of them were mapped in the gene and have only residual activity of its product, -acetylserine sulphydrylase A [-acetyl--serine acetate-lyase (adding hydrogen-sulphide); EC 4.2.99.8]. Sensitivity of wild type and mutants to azide was also reversed by a constitutive mutation in , the regulatory gene of cysteine biosynthesis. and mutants showed cross-resistance to azide and 1,2,4-triazole. It is suggested that the resistance of these mutants to azide is due to an increased activity of NADPH-sulphite reductase.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-113-1-45
1979-07-01
2021-05-13
Loading full text...

Full text loading...

/deliver/fulltext/micro/113/1/mic-113-1-45.html?itemId=/content/journal/micro/10.1099/00221287-113-1-45&mimeType=html&fmt=ahah

References

  1. Bogucka K., Wojtczak L. 1966; Effect of sodium azide on oxidation and phosphorylation processes in rat-liver mitochondria. Biochimica et biophysica acta 122:381–392
    [Google Scholar]
  2. Caughey W.S., Wallace W.J., Volpe J.A., Yoshikawa S. 1976; Cytochrome c oxidase. In The Enzymes 13 pp. 299–361 Boyer P.D. Edited by New York: Academic Press.;
    [Google Scholar]
  3. Cieśla Z., Bagdasarian M., Szczurkiewicz W., Przygonska M., Kłopotowski T. 1972; Defective cell division in the thermosensitive mutants of Salmonella typhimurium. . Molecular and General Genetics 116:107–125
    [Google Scholar]
  4. Cieśla Z., Mardarowicz K., Kłopotowski T. 1974; Inhibition of DNA synthesis and cell division in Salmonella typhimurium by azide. Molecular and General Genetics 135:339–348
    [Google Scholar]
  5. Dreyfuss J., Monty K.J. 1963; The biochemical characterization of cysteine-requiring mutants of Salmonella typhimurium. . Journal of Biological Chemistry 238:1019–1031
    [Google Scholar]
  6. Fimmel A.L., Loughlin R.E. 1977; Isolation and characterization of cysK mutants of Escherichia coli K12. Journal of General Microbiology 103:37–43
    [Google Scholar]
  7. Gornall A.G., Bardawill D.J., David M.M. 1949; Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry 177:751–766
    [Google Scholar]
  8. Harold F.M. 1972; Conservation and transformation of energy by bacterial membranes. Bacteriological Reviews 36:751–766
    [Google Scholar]
  9. Hulanicka M.D., Kłopotowski T. 1972; Mutants of Salmonella typhimurium resistant to triazole. Acta biochimica polonica 19:27–32
    [Google Scholar]
  10. Hulanicka M.D., Kłopotowski T., Smith D.A. 1972; The effect of triazole on cysteine biosynthesis in Salmonella typhimurium. . Journal of General Microbiology 72:291–301
    [Google Scholar]
  11. Hulanicka M.D., Kredich N.M., Treiman D.M. 1974; The structural gene for O-acetylserine sulphydrylase A in Salmonella typhimurium: identity with TrzA locus. Journal of Biological Chemistry 249:867–872
    [Google Scholar]
  12. Jones-Mortimer M.C. 1968a; Positive control of sulphate reduction in Escherichia coli. Isolation, characterization and mapping of cysteineless mutants of Escherichia coli K12. Biochemical Journal 110:589–595
    [Google Scholar]
  13. Jones-Mortimer M.C. 1968b; Positive control of sulphate reduction in Escherichia coli. The nature of the pleiotropic cysteineless mutants of Escherichia coli K12. Biochemical Journal 110:597–602
    [Google Scholar]
  14. Kobayashi H., Maeda M., Anraku Y. 1977; Membrane-bound adenosine triphosphatase of Escherichia coli. III. Effects of sodium azide on the enzyme functions. Journal of Biochemistry 81:1071–1077
    [Google Scholar]
  15. Kovač L., Istenesova B. 1968; Oxidative phosphorylation in yeast. I. Isolation and properties of phosphorylating mitochondria from stationary phase cells. Biochimica et biophysica acta 153:32–42
    [Google Scholar]
  16. Kredich N.M. 1971; Regulation of l-cysteine biosynthesis in Salmonella typhimurium. Effect of growth on varying sulfur source and O-acetylserine on gene expression. Journal of Biological Chemistry 246:3474–3484
    [Google Scholar]
  17. Kredich N.M., Becker M.A., Tomkins G.M. 1969; Purification and characterization of cysteine synthetase, a bifunctional complex, from Salmonella typhimurium. . Journal of Biological Chemistry 244:2428–2439
    [Google Scholar]
  18. Kredich N.M., Foote L.J., Hulanicka M.D. 1975; Studies on the mechanism of inhibition of Salmonella typhimurium by 1,2,4-triazole. Journal of Biological Chemistry 250:7324–7329
    [Google Scholar]
  19. Loper J.C., Grabnar M., Stahl R.C., Hartman Z., Hartman P.E. 1964; Genes and proteins involved in histidine biosynthesis of Salmonella typhimurium. . Brookhaven Symposia in Biology 17:15–53
    [Google Scholar]
  20. Mccann J., Choi E., Yamasaki E., Ames B.N. 1975; Detection of carcinogens as mutagens in the Salmonella typhimurium microsome test: assay of 300 chemicals. Proceedings of the National Academy of Sciences of the United States of America 725135–5139
    [Google Scholar]
  21. Murphy M.J., Siegel L.M. 1973; Siroheme and sirohydrochlorin. Journal of Biological Chemistry 248:6911–6919
    [Google Scholar]
  22. Nilan R.A., Sideris E.G., Kleinhofs A., Sander C., Konzak C.F. 1973; Azide - a potent mutagen. Mutation Research 17:142–144
    [Google Scholar]
  23. Quereshi M.A., Smith D.A., Kingsman A.J. 1975; Mutants of Salmonella typhimurium responding to cysteine or methionine: their nature and possible role in the regulation of cysteine biosynthesis. Journal of General Microbiology 89:358–370
    [Google Scholar]
  24. Roisin M.P., Kepes A. 1972; The membrane ATP-ase of Escherichia coli. I. Ion dependence and ADP-ATP exchange reaction. Biochimica et biophysica acta 275:333–345
    [Google Scholar]
  25. Sakami W., Toennies G. 1942; The investigation of amino acid reactions by methods of non-aqueous titrimetry. II. Differential acetylation of hydroxy groups and a method for the prepara-tion of the O-acetyl derivatives of hydroxy amino acids. Journal of Biological Chemistry 144:203–217
    [Google Scholar]
  26. Schonbaum G.R., Chance B. 1976; Catalase. In The Enzymes 13 pp. 363–408 Boyer P.D. Edited by New York: Academic Press.;
    [Google Scholar]
  27. Siegel L.M., Kamin H. 1971a; TPNH-sulfite reductase (Escherichia coli). Methods in Enzymology 17B:539–545
    [Google Scholar]
  28. Siegel L.M., Kamin H. 1971b; TPNH-sulfite reductase (SiR) from E. coli and Salmonella typhimurium: subunit structure and gene assignment. Federation Proceedings 301261
    [Google Scholar]
  29. Siegel L.M., Davis P.S., Kamin H. 1974; Reduced nicotinamide adenine dinucleotide phosphate-sulfite reductase of enterobacteria. III. The Escherichia coli hemoflavoprotein: catalytic parameters and the sequence of electron flow. Journal of Biological Chemistry 249:1572–1586
    [Google Scholar]
  30. Smith D.A. 1971; S-amino acids metabolism and its regulation in Escherichia coli and Salmonella typhimurium. . Advances in Genetics 16:141–165
    [Google Scholar]
  31. Smith H.O., Levine M. 1967; A phage P22 gene controlling integration of prophage. Virology 31:207–216
    [Google Scholar]
  32. Spencer H.T., Collins J., Monty K.J. 1967; Sequential regulation of cysteine biosynthesis in Salmonella typhimurium. . Federation Proceedings 26677
    [Google Scholar]
  33. Vogel H.J., Bonner D.M. 1956; Acetylornithinase of Escherichia coli: partial purification and some properties. Journal of Biological Chemistry 218:97–106
    [Google Scholar]
  34. Yoshimoto A., Sato R. 1968; Studies on yeast sulfite reductase. I. Purification and characterization. Biochimica et biophysica acta 153:555–575
    [Google Scholar]
  35. Yura Z., Wada C. 1968; Phenethyl alcohol resistance in Escherichia coli. I. Resistance of strain C600 and its relation to azide-resistant strain. Genetics 59:177–190
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-113-1-45
Loading
/content/journal/micro/10.1099/00221287-113-1-45
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error