Acid RNAases of ST were resolved into one minor and two major peaks by Sulphopropyl Sephadex chromatography. The same three peaks were obtained from exponentially growing cells and from heat-synchronized cells. The two major RNAases (peaks 2 and 3) were also separated by polyacrylamide gel electrophoresis at pH 4.5 and 8.3. The stepwise increase in total RNAase activity during synchronous growth was primarily caused by a large and preferential increase in the activity of the peak 3 RNAase during the first hour after the heat shock.


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