1887

Abstract

Intrastrand self-complementary sequences have been isolated from the DNA of by hydroxyapatite (HA) chromatography following thermal renaturation of strands separated by chromatography on methylated albumin-kieselguhr (MAK). The intrastrand structures derived from the MAK H strand (HA HII) were biologically active showing transforming activity for a wide variety of markers, as well as hybridization to both pulse-labelled and ribosomal RNA. Removal of regions of single-strand DNA with S1 nuclease did not significantly alter the biological activity of the self-annealed molecules. The overall efficiency of transformation and hybridization of the intrastrand self-annealing DNA was low suggesting that many sequences in the population are neither active in transformation to prototrophy nor transcribed into RNA.

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1979-04-01
2021-07-30
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