1887

Abstract

Summary: Intrastrand self-complementary sequences have been isolated from the DNA of by hydroxyapatite (HA) chromatography following thermal renaturation of strands separated by chromatography on methylated albumin-kieselguhr (MAK). The intrastrand structures derived from the MAK H strand (HA HII) were biologically active showing transforming activity for a wide variety of markers, as well as hybridization to both pulse-labelled and ribosomal RNA. Removal of regions of single-strand DNA with S1 nuclease did not significantly alter the biological activity of the self-annealed molecules. The overall efficiency of transformation and hybridization of the intrastrand self-annealing DNA was low suggesting that many sequences in the population are neither active in transformation to prototrophy nor transcribed into RNA.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-111-2-353
1979-04-01
2019-10-19
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-111-2-353
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error