%0 Journal Article %A Galloway, Denise A. %A Rudner, Rivka %T Intrastrand Self-complementary Sequences in Bacillus subtilis DNA %D 1979 %J Microbiology, %V 111 %N 2 %P 353-361 %@ 1465-2080 %R https://doi.org/10.1099/00221287-111-2-353 %I Microbiology Society, %X Intrastrand self-complementary sequences have been isolated from the DNA of Bacillus subtilis by hydroxyapatite (HA) chromatography following thermal renaturation of strands separated by chromatography on methylated albumin-kieselguhr (MAK). The intrastrand structures derived from the MAK H strand (HA HII) were biologically active showing transforming activity for a wide variety of markers, as well as hybridization to both pulse-labelled and ribosomal RNA. Removal of regions of single-strand DNA with S1 nuclease did not significantly alter the biological activity of the self-annealed molecules. The overall efficiency of transformation and hybridization of the intrastrand self-annealing DNA was low suggesting that many sequences in the population are neither active in transformation to prototrophy nor transcribed into RNA. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-111-2-353