1887

Abstract

Summary: The effect of phenol, mercuric chloride, fluoride, bisulphite, azide, malonate, 2:4-dinitrophenol and cyanide on respiration and penicillin production was studied using suspensions of washed penicillin-producing mycelium of in Warburg flasks. Inhibition of penicillin production by phenol and by mercuric chloride closely followed the inhibition of respiration. Both fluoride and bisulphite inhibited penicillin production to a greater extent than respiration. Malonate was without effect on respiration or penicillin production, possibly because the pH was too high to allow a sufficient concentration of undissociated acid. Penicillin production was inhibited by 2:4-dinitrophenol at concentrations which had little effect on respiration, suggesting that phosphate bond energy is utilized in penicillin formation. Penicillin production was extremely sensitive to cyanide. A concentration of 0·000002 -KCN, which caused no significant inhibition of respiration, depressed the rate of penicillin production to . 25% of normal. Maximum rates of penicillin production and respiration are dependent on cyanide-sensitive systems but an alternative, relatively cyanide-stable system allows both respiration and penicillin production to proceed at 25% of the maximum rate.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-11-3-412
1954-12-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/micro/11/3/mic-11-3-412.html?itemId=/content/journal/micro/10.1099/00221287-11-3-412&mimeType=html&fmt=ahah

References

  1. Clifton C.E. 1946; Microbial assimilations. Advanc. Enzymol. 6:269
    [Google Scholar]
  2. Fowler C.B. 1951; The relationship between fermentation and enzymatic adaptation. Biochim. Biophys. Acta 7:563
    [Google Scholar]
  3. Frantz I.D., Zamecnik P.C., Reese J.W., Stephenson M.L. 1948; The effect of dinitrophenol on the incorporation of alanine labelled with radioactive carbon into the proteins of slices of normal and malignant rat liver. J. biol. Chem. 174:773
    [Google Scholar]
  4. Hotchkiss R.D. 1947; The assimilation of amino acids by respiring washed staphylococci. Fed. Proc. 6:263
    [Google Scholar]
  5. Hugo W.B., Street H.E. 1952; The effect of phenol, 2-phenoxyethanol and cetyltrimethylammonium bromide on the oxidation of various substrates by Escherichia coli. J. gen. Microbiol. 6:90
    [Google Scholar]
  6. Jarvis F.G., Johnson M.J. 1950; The mineral nutrition of Penicillium chrysogenum Q176. J. Bact. 59:51
    [Google Scholar]
  7. Loomis W.F., Lipmann F. 1948; Reversible inhibition of the coupling between phosphorylation and oxidation. J. biol. Chem. 173:807
    [Google Scholar]
  8. Stevens C.M., Vohra P., Inamine E., Roholt O.A. 1953; Utilisation of sulphur compounds for the biosynthesis of penicillins. J. biol. Chem. 205:1001
    [Google Scholar]
  9. Turner J.S., Hanly V. 1947; Malonate and plant respiration. Nature; Lond.: 160:296
    [Google Scholar]
  10. Umbreit W.W., Burris R.H., Stauffer J.F. 1945 Manometric Techniques and Related Methods for the Study of Tissue Metabolism. Minneapolis, Minn. U.S.A.: Burgess Publishing Co.;
    [Google Scholar]
/content/journal/micro/10.1099/00221287-11-3-412
Loading
/content/journal/micro/10.1099/00221287-11-3-412
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error