1887

Abstract

The influence of amino acids on the synthesis of staphylococcal serine-proteinase (proteinase I) by strain v8 was studied. The repression of proteinase synthesis during the exponential growth phase was not due to any single amino acid in the medium. Proline was essential for proteinase synthesis in post-exponential growth but not for bacterial growth. At low concentrations of glycine, serine, threonine and proline, proteinase synthesis was inhibited, whereas at high concentrations of any of these amino acids, proteinase synthesis was fully induced. During post-exponential growth in the absence of glycine, serine, threonine or proline, the intracellular concentrations of alanine, aspartic acid and glutamic acid increased two to threefold. In the presence of these amino acids, the pool of alanine and aspartic acid remained at a low level and the concentration of glutamic acid decreased. The synthesis of proteinase I is probably repressed at high intracellular concentrations of glutamic acid.

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1978-08-01
2024-11-13
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References

  1. Abbas-Ali B., Coleman G. 1977; The characteristics of extracellular protein secretion by Staphy-lococcus aureus (Wood 46) and their relationship to the regulation of alpha-toxin. Journal of General Microbiology 99:277–282
    [Google Scholar]
  2. Arvidson S. 1973a; Studies on extracellular proteolytic enzymes from Staphylococcus aureus. II. Isolation and characterization of an EDTA-sensitive protease. Biochimica et biophysica acta 302:149–157
    [Google Scholar]
  3. Arvidson S. 1973b; Hydrolysis of casein by three extracellular proteolytic enzymes from Staphylococcus aureus, strain v8. Acta pathologica et microbiologica scandinavica B81:538–544
    [Google Scholar]
  4. Arvidson S., Holme T., Lindholm B. 1972; The formation of extracellular proteolytic enzymes from Staphylococcus aureus. Acta pathologica et microbiologica scandinavica B80:835–844
    [Google Scholar]
  5. Arvidson S., Holme T., Lindholm B. 1973; Studies on extracellular proteolytic enzymes from Staphylococcus aureus. I. Purification and characterization of one neutral and one alkaline protease. Biochimica et biophysica acta 302:135–148
    [Google Scholar]
  6. Arvidson S., Björklind A., Eriksson R., Holme T. 1976; Enzymes from Staphylococcus aureus. In Continuous Culture: Applications and New FieldsProceedings of the 6th International Symposium on Continuous Culture of Microorganisms238–250 Dean A. C. R., Ellwood D. C., Evans C. G. T., Melling J. Chichester: Ellis Horwood;
    [Google Scholar]
  7. Björklind A., Arvidson S. 1977; Occurrence of an extracellular serine-proteinase among staphy-lococcal strains. Acta pathologica et microbiologica scandinavica B85:277–280
    [Google Scholar]
  8. Björklind A., Jörnvall H. 1974; Substrate specificity of three different extracellular proteo-lytic enzymes from Staphylococcus aureus. Biochimica et biophysica acta 370:524–529
    [Google Scholar]
  9. Borkowski J. D., Johnson M. J. 1967; Long-lived steam-sterilizable membrane probes for dissolved oxygen measurements. Biotechnology and Bioengineering 9:635–639
    [Google Scholar]
  10. Coleman G. 1967; Studies on the regulation of extracellular enzyme formation by Bacillus subtilis. Journal of General Microbiology 49:421–431
    [Google Scholar]
  11. Coleman G., Stormonth D. A. 1973; The relationship between the nucleotide pool of Bacillus amyloliquefaciens and the control and secretion of extracellular enzymes. Biochemical Society Transactions 1:394–397
    [Google Scholar]
  12. Dugas H., Gaudet F. 1975; Structural studies of staphylococcal protease. I. Spin labelling of the active site and a comparison with other proteases. Canadian Journal of Biochemistry 53:155–163
    [Google Scholar]
  13. Hancock R. 1958; The intracellular amino acids of Staphylococcus aureus: release and analysis. Biochimica et biophysica acta 28:402–412
    [Google Scholar]
  14. Heathcote J. G., Haworth C. 1969; An improved technique for the analysis of amino acids and related compounds on thin layers of cellulose. II. The quantitative determination of amino acids in protein hydrolysates. Journal of Chromatography 43:84–92
    [Google Scholar]
  15. Holme T., Arvidson S. 1976; Changes in Yo2 in relation to growth rate and production of extracellular enzymes by Staphylococcus aureus. Zentralblatt für Bakteriologie,Parasitenkunde,Infektionskrankheiten und Hygiene (Abteilung I) 5:181–188
    [Google Scholar]
  16. Houmard J., Drapeau G. R. 1972; Staphylococcal protease: a proteolytic enzyme specific for glutamoyl bonds. Proceedings of the National Academy of Sciences of the United States of America 693506–3509
    [Google Scholar]
  17. Landwall P., Holme T. 1977; The removal of inhibitors of bacterial growth by dialysis culture. Journal of General Microbiology 103:345–352
    [Google Scholar]
  18. Laurell C. B. 1966; Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Analytical Biochemistry 15:45–52
    [Google Scholar]
  19. Litchfield C. D., Prescott J. M. 1970; Regulation of proteolytic enzyme production by Aeromonas proteolytica. I. Extracellular endo-peptidase. Canadian Journal of Microbiology 16:17–22
    [Google Scholar]
  20. McDonald I. J., Chambers A. K. 1966; Regulation of proteinase formation in a species of Micrococcus. Canadian Journal of Microbiology 12:1175–1185
    [Google Scholar]
  21. Payne J. W., Gilvarg C. 1971; Peptide transport. Advances in Enzymology 35:187–244
    [Google Scholar]
  22. Pollock M. R. 1962; Exoenzymes. In The Bacteria 4121–178 Gunsalus I. C., Stanier R. Y. New York and London: Academic Press;
    [Google Scholar]
  23. Tanaka S., Iuchi S. 1971; Induction and repression of an extracellular proteinase in Vibrio parahemolyticus. Biken Journal 14:81–96
    [Google Scholar]
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