1887

Abstract

A number of deoxyribonucleoside-requiring mutants () of were isolated and their growth characteristics and ribonucleotide reductase activities were compared with those of the wild type and of a mutant (). Both and mutants required the presence of a mixture of deoxyribonucleosides for growth at 45 °C but not at 25 °C. All the mutant strains tested contained ribonucleotide reductase activity which showed heat sensitivity similar to that of the enzyme from a wild-type strain. The reductase in seemed to reduce ribonucleoside triphosphates in a similar manner to the enzyme in

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-107-1-139
1978-07-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/micro/107/1/mic-107-1-139.html?itemId=/content/journal/micro/10.1099/00221287-107-1-139&mimeType=html&fmt=ahah

References

  1. Bazill G. W., Karamata D. 1972; Tempera-ture-sensitive mutants of Bacillus subtilis defective in deoxyribonucleotide synthesis. Molecular and General Genetics 117:19–29
    [Google Scholar]
  2. Beck W. S., Levin M. 1963; Purification, kinetics and repression control of bacterial trans-N-deoxyribosylase. Journal of Biological Chemistry 238:702–709
    [Google Scholar]
  3. Elford H. 1968; Effect of hydroxyurea on ribonucleotide reductase. Biochemical and Biophysical Research Communications 33:129–135
    [Google Scholar]
  4. Fuchs J. A., Neuhard J. 1973; A mutant of Escherichia coli defective in ribonucleoside diphosphate reductase. European Journal of Biochemistry 32:451–456
    [Google Scholar]
  5. Fuchs J. A., Karlstrøm H. O., Warner H. R., Reichard P. 1972; Defective gene product in dnaF mutant of Escherichia coli. Nature New Biology 238:69–71
    [Google Scholar]
  6. Goulian M., Beck W. S. 1966; Purification and properties of a cobamide dependent ribonucleotide reductase from Lactobacillus leich-mannii. Journal of Biological Chemistry 241:4233–4242
    [Google Scholar]
  7. Karlstrøm H. O. 1970; Inability of Escherichia coli B to incorporate added deoxycytidine, deoxyadenosine and deoxyguanosine into DNA. European Journal of Biochemistry 11:68–71
    [Google Scholar]
  8. Larsson A., Reichard P. 1966; Enzymatic synthesis of deoxyribonucleotides. X. Reduction of purine ribonucleotides: allosteric behaviour and substrate specificity of the enzyme system from Escherichia coli B. Journal of Biological Chemistry 241:2540–2543
    [Google Scholar]
  9. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  10. O’Donovan G. A., Neuhard J. 1970; Pyri-midine metabolism in microorganisms. Bacteriological Reviews 34:278–343
    [Google Scholar]
  11. Rima B. K., Takahashi I. 1973; The synthesis of nucleic acids in Bacillus subtilis infected with phage PBS1. Canadian Journal of Biochemistry 51:1219–1224
    [Google Scholar]
  12. Rima B. K., Takahashi I. 1977; Metabolism of pyrimidine bases and nucleosides in Bacillus subtilis. Journal of Bacteriology 129:574–579
    [Google Scholar]
  13. Rima B. K., Takahashi I. 1978; Synthesis of thymidine nucleotides in Bacillus subtilis. Canadian Journal of Biochemistry 56:158–160
    [Google Scholar]
  14. Sinha N. K., Snustadt D. P. 1972; Mechanism of inhibition of deoxyribonucleic acid synthesis in Escherichia coli by hydroxyurea. Journal of Bacteriology 112:1321–1334
    [Google Scholar]
  15. Spizizen J. 1958; Transformation of biochemically deficient strains of Bacillus subtilis by deoxy-ribonucleate. Proceedings of the National Academy of Sciences of the United States of America 441072–1078
    [Google Scholar]
  16. Tomita F., Takahashi I. 1976; Changes in enzyme activities in Bacillus subtilis infected with bacteriophage PBS1. In Microbiology -1976315–318 Schles-singer D. Washington: American Society for Microbiology;
    [Google Scholar]
  17. Wilson M. C., Farmer J. L., Rothman F. 1966; Thymidylate synthesis and aminopterin resistance in Bacillus subtilis. Journal of Bacteriology 92:186–196
    [Google Scholar]
  18. Yau S., Wachsman J. T. 1973; The Bacillus megaterium ribonucleotide reductase: evidence for a B12 coenzyme requirement. Molecular and Cellular Biochemistry 1:101–105
    [Google Scholar]
/content/journal/micro/10.1099/00221287-107-1-139
Loading
/content/journal/micro/10.1099/00221287-107-1-139
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error