SUMMARY: A number of deoxyribonucleoside-requiring mutants () of were isolated and their growth characteristics and ribonucleotide reductase activities were compared with those of the wild type and of a mutant (). Both and mutants required the presence of a mixture of deoxyribonucleosides for growth at 45 C but not at 25 C. All the mutant strains tested contained ribonucleotide reductase activity which showed heat sensitivity similar to that of the enzyme from a wild-type strain. The reductase in seemed to reduce ribonucleoside triphosphates in a similar manner to the enzyme in


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